Study On Preparation And Characterization Of Curcumin Nanoliposome

Curcumin is one of the main active ingredients of turmeric,and it possesses variety of physiological functions such as antioxidant,anti-inflammatory,anti-viral,anti-bacterial,anti-tumor and so on.However,curcumin has low solubility in water,and it is unstable in neutral to slightly alkaline environment and sensitive to light,heat and metal ions,besides,curcumin has low bioavailability after metabolism,which all greatly limit the application of curcumin.Liposome encapsulation technology is a targeted delivery technology and emerging in recent years,which has a wide range of encapsulation and can improve the solubility,stability and physiological activity of embedded objects.In order to improve the stability and physiological activity of curcumin,curcumin nanoliposome was prepared,and the physical and chemical properties,stability,antioxidant activity,cytotoxicity and cell uptake rate of curcumin nanoliposome were studied.Preparation and characterization of physical and chemical properties of curcumin nanoliposome:reverse evaporation combined with dynamic high pressure microfluidization?DHPM?was used to prepare curcumin nanoliposome.The encapsulation efficiency was 57.1±1.1%,particle size was 68.1±1.5nm,dispersion coefficient was 0.246,potential was-3.16±0.34mV and the appearance of curcumin nanoliposome was a uniform morphology of spherical or ellipsoidal.FTIR spectra confirmed the role of liposome that entrapped curcumin.Compared with free curcumin,curcumin nanoliposome had good sustained release properties?the release was 63.1±5.1%and 96.2±1.5%respectively at 24h?.Stability of curcumin nanoliposome:The stability at pH 6.5 of both nanoliposome and curcumin was good.At pH 7.4,8.0 and 10.0,the stability of the curcumin nanoliposome was significantly higher than of curcumin.But with the pH rising to 12.0,the protection of nanoliposome for curcumin significantly decreased.Except in Li⁺solution,nanoliposome encapsulation significantly improved the stability of curcumin in Fe³⁺,Al³⁺,Fe²⁺,Cu²⁺and K⁺ions solution.Free curcumin and curcumin nanoliposome were stable in SGF environment.Nanoliposome significantly improved the stability of curcumin in SIF environment?there were 41.0±3.4%and6.6±2.7%curcumin remaining after 3h testing,respectively?.Curcumin nanoliposome had good physical stability during storage at 4?.While stored at 25?,the particle size and zeta potential of liposome were significantly higher,and the encapsulation efficiency?EE?significantly reduced.Bioavailability of curcumin nanoliposome:Curcumin nanoliposome well preserved the in vitro and cellular antioxidant activity of curcumin.But liposome-entrapped significantly reduced the toxicity of curcumin to CaCo-2 cells?when treated with 100?g/mL of samples,there were 32.8±4.6%and 88.5±4.3%of Caco-2 cells apoptosis,respectively?.The cellular uptake of curcumin nanoliposome was significantly lower than free curcumin?the cell uptake at 4 h were 69.6±5.4%and 92.9±3.6%,respectively?.