Study On Spatial And Temporal Distribution And Halophilic Bacteria Diversity In Panjin Wetland

As a common microorganism in extreme environment,halophilic bacteria exist in high salt lake wetland and other environments.In recent years,with the continuous isolation of new halophilic bacteria,the exploration of their functions is also in progress.The systematic study of halophilic bacteria is an important foundation to reveal its function of regulating high salt wetland and improving the poor ecological environment.Panjin Wetland covers an area of about 315000 hectares.It is a high salt and alkali area with high salt content.At the same time,it plays an important role in regulating the surrounding ecological environment.Based on this,systematic research on the composition of halophilic bacteria in Panjin Wetland is an important entry point to comprehensively reveal the biological activity of the wetland.In recent years,people have been exploring halophilic bacteria,not only isolating many new halophilic bacteria,but also finding out the application prospect and value of halophilic bacteria with the continuous exploration and discovery,the continuous understanding of strains.Halophilic bacteria are widely distributed under extremely harsh conditions,among which there are many halophilic archaea,which play an important role in the application of biotechnology and the exploration of high salt lake wetland.In this experiment,laboratory culture and 16 S RNA sequencing of microorganisms were used to study and analyze halophilic microorganisms in Panjin Wetland.By comparing the soil physical and chemical properties,microbial population structure and related physical and chemical properties of different plant microorganisms in different seasons and depths,the main conclusions are as follows:(1)In April,July and November,the soil water content under the cover of Suaeda salsa was larger than that of reed,and the water content under the soil of reed and Suaeda salsa showed a trend of increasing first and then decreasing with the increase of soil depth,while it did not show a significant change trend under the blank control.Due to precipitation and other reasons,the soil moisture content under reed and Suaeda salsa was the highest in July,the second in April and the lowest in November.The soil in this area is alkaline,and the pH value is basically between7.9-9.In July,the pH value of the soil is the lowest.The salinity of soil under Suaeda salsa is 176.20-341.50mg/kg,while the content of chloride ion under reed is146.96-335.18mg/kg,and the content of chloride ion in non plant growing soil is189.07-335.21mg/kg.The content of chloride ion in the three soil types decreased first and then increased.In the three seasons,the content of ammonia nitrogen in reed and Suaeda salsa soil was the highest in April.(2)There are 32 phyla,287 families and 683 genera in Suaeda salsa in three seasons.Among them,Proteobacteria is the most dominant bacteria;actinobacteria and bacteria unclassified are the dominant genera under different soil depths in different seasons.Compared with inorganic salts,the composition of organic matter is more closely related to the main composition of bacteria in the environment.Virgibacillus and alkalibacillus are two genera which have great influence on the soil and the microbial community under the environment of Suaeda salsa.Its abundance is closely related to the content of inorganic salt and organic matter in the results.(3)In this paper,the bacteria and microorganisms in Suaeda salsa soil were screened,isolated and cultured.Cm medium was used to screen and re screen the strains,and six strains were screened finally.The carbon source and some physical and chemical properties of the six strains were tested.The first generation sequencing of the six sequenced strains was carried out.Combined with the phylogenetic tree analysis,the identification of strain Q12,virgibaci,was obtained llus Kapii ? Q31 species is virgibacillus dokdonensis.The 16 S rRNA of strain C4 is alkalicacillus almalahensis,the strain X21 is alkalicacillus salilacus,the 16 S of strain x27 is Halomonas sinaiensis,and the species of C34 is Halomonas denitrificans,all of which are halophilic and alkali resistant.