Effects Of Amylin On Food Intake Via Intercostals Sympathetic Innervation Of The Interscapular Brown Adipose Tissue

Obesity is cased by over deposition of fat rely on the interaction between central neuron projection and sympathetic nerve.Amylin acts to anorexic,weight-loss effects and activates thermogenesis in the interscapular brown adipose tissue(IBAT)cross the blood-brain barrier(BBB),play a significant role in alleviate obesity.Given the postion effect of SNS and the anorexic of Amylin,we consider whether signal-regulated kinase(ERK),nNOS,and ChAT activity in the LDT are responsible for amylin's anorexigenic effects,and whether this requires an intact SNS.C57BL/6J male mice[6-8 weeks,wild-type(WT),sham,and sympathetic denervation of IBAT]were used.Food consumption,body weight,Insulin and Glucose,the distribution of pERK,nNOS,and ChAT positive neurons in the brain were examined following acute and chronic amylin administration(50?g/kg).The results showed that food intake was significantly decreased in WT and sham mice following amylin injection,but not in the denervated mice at 4h.Amylin fail to increased the expression of nNOS,ChAT and pERk in LDT in the denervated mice,consistent result in western blot.Amylin increased the expression of UCP-1 mRNA and protein in WT and sham mice,but not Denervation.Chronic amylin reduced serum glucose levels after 6 weeks no 4 h,but increased insulin levels,no change was observed in denervated mice.Overall,the current study demonstrated that amylin regulation of food intake and activates UCP1 was highly dependent on the intact SNS.And the pERK,nNOS.and ChAT activated by amylin regulates in the metabolic process.