Effect Of Amylin On Steroidogenic Regulatory Factors In Central Nervous System Of Mouse

As a 37 amino acid polypeptide secreted by islet ? cells.Amylin can participate in the central nervous system through the blood-brain barrier and participate in the dual role of suppressing food and promoting temperature,and can also participate in reproductive regulation indirectly.In view of the fact that steroid hormones play an active role in maintaining body organ development,energy metabolism,reproduction and other processes,their synthesis and secretion are regulated by the central nervous system of the brain.Therefore,it is hypothesized that amylin regulates testosterone secretion through central nervous system steroid synthesis regulators(3?-HSD1,CYP17A1 and StAR)and is involved in body weight regulation.The distribution of steroid hormone synthesis regulators in the central nervous system of mice was detected by immunofluorescence,and amylin was used to detect the steroid hormone synthesis regulator and pERK protein in the central nervous system of mice.The expression changes,presumably its effect on the secretion of androgen(testosterone).The results showed that the neurons of steroid synthesis regulators were distributed in many nucleus regions of the central nervous system of mice,and the 3?-HSD1 and CYP17A1 factors were widely expressed,while the StAR neural signals were mainly distributed in the indeterminate zone of the thalamus(ZI).In the locus coeruleus(LC),it was scattered;in addition,intraperitoneal injection of amylin for 11 days significantly reduced body weight(p<0.05)and serum testosterone levels(p<0.01),significantly inhibited the expression of steroidogenic regulators in the hypothalamus,and promoted pERK protein expression(p<0.01).These results suggest that intraperitoneal injection of amylin can inhibit the expression of steroidogenic regulatory factors neurons and reduce serum testosterone levels through the pERK signaling pathway.At the same time,it shows that amylin can interfere with the secretion of androgen(testosterone)and participate in reproductive regulation in addition to the dual effects of suppressing food and promoting temperature.