Effects Of Nitrogen Source On Fatty Acids And Stable Isotope Compositions Of Suaeda Heteroptera

In this paper,we selected Suaeda heteroptera as the research object to study the effect of nitrogen source on Liaohekou wetland plants.In order to realize the effect of different nitrogen source concentrations on the physiological and ecological aspects of Suaeda heteroptera,the content of carbohydrates,proteins,lipids,and fatty acids in the aboveground and underground tissues(roots,stems,and seeds)of Suaeda heteroptera in different growth periods were measured and analyzed.When determining the carbohydrate content of Suaeda heteroptera,the orthogonal test was used to optimize the determination and extraction process of carbohydrates,and the optimal experimental conditions.were selected for.determination and analysis.Stable isotope technology is also used in this paper to explore the effects of different levels of nitrogen source concentration on the carbon stable isotope composition of fatty acids of Suaeda heteroptera.It provides a theoretical basis for further research on nitrogen sources for the Liaohekou wetland ecosystem.The research conclusion is as follows:Based on the analysis of the determination results of various substances in Suaeda heteroptera,it was found that with the increase of the nitrogen source concentration,the carbohydrate content in Suaeda heteroptera roots and seeds increased significantly;in terms of protein content,it significantly increased in the seeds of Suaeda heteroptera,which showed an initial increase and a late decrease in the roots and stems;in terms of fatty acid content,the roots,stems,and seeds of Suaeda heteroptera showed an increasing trend..Using GC-MS technology to analyze the fatty acid content of Suaeda heteroptera,the results showed that with the increase of nitrogen source concentration,the fatty acid content of Suaeda heteroptera root increased in May and July,and decreased in June and September;the content of unsaturated fatty acids increased in the middle of the May,while the content of other fatty acids decreased in May.The fatty acid content increased first and then decreased in June.The fatty acid content increased gradually in July and September.The fatty acid content in seeds increased.Regarding the analysis of the stable carbon isotope composition of the fatty acids of Suaeda heteroptera,we have concluded that the carbon stable isotope composition of various fatty acid carbons in different periods will change due to changes in the concentration of the nitrogen source.In Suaeda heteroptera roots,the saturated fatty acids 813C18:0,?13C21:0 and?13C23:0 changed significantly,especially C21:0 had the most negative ?13C value under high nitrogen treatment.Compared with the control group,the ?13CFAs in the low-nitrogen and high-nitrogen treatment groups were negative compared to the control group.At the same time,the stable carbon isotope composition of saturated fatty acids(C22:o and C24:0)is greatly affected by the nitrogen source concentration.Except for September,?13C22:0 and ?13C24:0 are the lowest at low nitrogen concentrations in other periods.The variation range of ?13CfAs of seeds in November was-42.337?-32.085%?,of which ?13C18:2,?13C18:1,?13C22:o and ?13C24:0 changed significantly,except that ?13C18:2 was negative under high nitrogen concentration,the other 813C values were lower in the control group than the treatment group.It showed that the change of nitrogen source concentration had a greater influence on the ?13C value of saturated fatty acids in different tissues of Suaeda heteroptera.Through the above studies,the effects of different levels of nitrogen source on the aboveground and underground plant tissues of Suaeda heteroptera were analyzed,which provided a certain theoretical basis for the ecological protection and restoration of wetlands.