Study Of The Diversity Of The Rhizospheric Bacteria And The Load Capacity To Oil Degrading Bacteria Of Suaeda Heteroptera

This study collected Suaeda Heteroptera Kitag from Delta of Panjin Liaoning Province, the bacterial diversity in rhizosphere soil and root homogenate of Suaeda Heteroptera Kitag was analyzed by traditional culture methods and high-throughput sequencing. Two methods would be used to extract the total DNA to obtain samples of eight different sites root and soil microbial genome and culturable bacterial genome, using high-throughput sequencing method of 16 srDNA sequence of bioinformatics analysis, determination of Shannon diversity index and community structure. The selected strains were identified by morphological observation and analysis of 16 S rDNA sequences. The oil-degradation bacteria which isolated from rhizosphere of Suaeda Heteroptera Kitag would be used to soak the weed to determine the load capacity to oil degrading bacteria of Suaeda Heteroptera Kitag. The results showed that the bacterial diversity in rhizosphere soil is higher than root homogenate of Suaeda Heteroptera Kitag. In total, thirty phyla were identified from these samples, which were Acidobacteria, Actinobacteria, Aminicenantes, Bacteroidetes, Chlorobi, Chloroflexi, Cloacimonetes, Cyanobacteria, Deferribacteres, Deinococcus-Thermus, Elusimicrobia, Fibrobacteres, Firmicutes, Fusobacteria, Gemmatimonadetes, Gracilibacteria, Hydrogenedentes, Latescibacteria, Lentisphaerae, Marinimicrobia, Nitrospirae, Omnitrophica, Parcubacteria, Planctomycetes, Proteobacteria, Saccharibacteria, Spirochaetes, Tenericutes, Thermotogae, Verrucomicrobia.The rhizosphere soil samples with highest bacterial diversity were, in order, S4-2T,S1-1T and S3 T.In the endophytic bacteria samples, the highest diversity index is S4-3G, the second is S2-2G, the lowest is S4-1G.Four hundred and five strains were isolated from these eight samples(S1-1, S1-2, S2-1, S2-2, S3, S4-1, S4-2, S4-3)with traditonal methods.The selected strains were identified by morphological observation and analysis of 16 S rDNA sequences and physio-biochemical characteristics. The results showed that the strains were mainly affiliated with the genus Idiomarina(18), Marinomonas(110), Psychrobacter(62), Sphingomonas(10), Pseudoalteromonas(39), Vibrio(42), Halomonas(18), Brevibacterium(4), Arthrobacter(3),Salinicola(3),Bacillus(24), Planococcus(5), Altererythrobacter(6), Brevundimonas(2), Shewanella(3), Oceanisphaera(4), Marinobacterium(5), Streptomyces(2), Pseudomonas(12),Microbacterium(5),Paracoccus(7), Zhihengliuella(7), Kushneria(2), ’Salinicola(3), Microbulbifer(6), Serratia(3). The microbial community structure rhizosphere soil and root homogenate of different Suaeda Heteroptera Kitag was different.The diversity of rhizospheric bacteria of Suaeda Heteroptera Kitag is high.The isolation rate of Marinomonas was 27%. Psychrobacter, Vibrio, Pseudoalteromonas, Halomonas and Bacillus were widely distributed, and these genus isolation rate was between 4%-10%. The most isolated strains(76) was S1-1, and the highest bacterial diversity was S1-2 which the main constituent were twenty-two genuses and thirty-two species. The lowest bacterial diversity was S4-3 which the main constituent were nine genuses and fourteen species.The optimum conditions of load capacity to oil degrading bacteria of Suaeda Heteroptera was bacteria concentrations of 105cell/mL and soaking time of 2h. The bacterial number would be 106cell/g when the Suaeda Heteroptera Kitag grew to the size at between 1 and 2 cm. Tissue section of root showed that the bacteria existed in the local cells of cork and secondary phloem in the root.