Genetic Diversity Of Captive Rhesus Macaques In Zoos In China

The zoo is a base for the education of the public on biodiversity and nature conservation.The rhesus macaques is one of the most popular species for tourists.The genetic background and population viability of the rhesus macaque should also be concerned.In this experiment,the mitochondrial D-loop region was used to evaluate the genetic diversity of the captive breeding rhesus macaques,we trying to use the COI and COII genes as rhesus monkey subspecies DNA barcodes at the molecular level to identified the macaque monkeys subspecies under captive environment.Sub-categories are classified as follows:1)Captive breeding populations in the zoo have a high level of genetic diversity as a whole(haplotype diversity Hd: 0.98,nucleotide diversity Pi: 0.06311).Of the 11 captive breeding populations,the Tibetan zoo had the lowest level of genetic diversity.This may be due to the fact that the rhesus macaques of Tibetan captive breeding population has a single source and has produced some inbreeding.2)Our results support that the Chinese rhesus macaques are mainly divided into two major branches.The mitochondrial haplotypes of rhesus monkeys in each zoo are embedded in caled A and B,indicating that the rhesus macaques have complex origin.3)The COI and COII genes of rhesus macaques subspecies both have some genetic variation,and there is a subspecies DNA barcode interval.The phylogenetic tree constructed by the subspecies COII gene cannot form obvious subspecies clustering,but the COI gene is constructed.The phylogenetic tree can form a clear cluster,and the COI gene is more suitable for use as a DNA barcode in rhesus macaques subspecies identification.4)When using the COI gene of rhesus macaques subspecies as the DNA barcode of the subspecies,the haplotype clustered with the subspecies is considered to have the pedigree of that subspecies.