Transcriptome And Correlative Relationship Of The Aphid Schlechtendalia Chinensis And Its Unique Primary Host Plant Rhus Chinensis

Schlechtendalia chinensis(Hemiptera: Aphididae: Eriosomatinae:Fordini)is one of the Rhus gall aphids,which only use Rhus chinensis Mill(Anacardiaceae)as its primary host plant.This species lives on the species R.chinensis to form galls,which is rich in tannins and widely applied in various field,e.g.medicine,printing and dyeing,petroleum exploration,rare metal extraction and food engineering.R.chinensis Mill is an important economic tree and also a herb plant.So far,the research on S.chinensis and Rhus chinensis Mill were mainly focused on classification and identification,growth and development,genetic breeding and phylogeny.However,the research about transcriptome and correlation between S.chinensis and R.chinensis Mill in transcript level has not been reported.Here,we sequenced the transcriptomics of S.chinensis and R.chinensis Mill by the high-throughput sequencing method and analyzed the correlation between them in transcript level.The results are as follows:1.Transcriptome of Schlechtendalia chinensisThree aphid treatments were deeply sequenced by Illumina platform and 46,516 Unigenes were obtained.By GO function analyzing,74% of differential gene were annotated.Supposedly,26% of differential gene which can not be annotated were new genes.Biologically-related regulatory genes occupied 53.37%,and were mainly related to reproductive gene.The differential gene of S.chinensis were mainly enriched in two pathways associated with cancer and neurodegenerative diseases.2.Transcriptome of Rhus chinensis MillFive Rhus chinensis samples using Illumina platform were deeply sequenced and resulted in a total of 48,910 Unigenes.Through the GO function analyzing,about 73% of differential gene were annotated and47.47% genes were related to the regulation of R.chinensis Mill biology characteristics.However,there were no genes found to relate to reproduction.There were 14115 SSR loci and 681239 SNP loci were identified,andapproximately 28.86% of Unigenes contained SSR loci.The transcripts mainly correlated with the following metabolic pathways: carbohydrate metabolism,amino acid metabolism,and energy metabolism.Among all GO pathways,1,6-diphosphate fructasewas found,that plays a key role in the heterogeneous metabolism of sugar and the synthesis of photosynthesis sucrose.3.Correlation between functional genes of S.chinensis and Rhus chinensis MillThrough the KEGG pathway enrichment and phylogenetic clustering trees,it was found that the genes corresponding to the three metabolic pathways of carbohydrate metabolism,amino acid metabolism and energy metabolism were more highly expressed in the R.chinensis Mill samples than the S.chinensis materials.The ML trees were constructed by using the similar genes of the S.chinensis and R.chinensis Mill,and the results shown that the growth of S.chinensis was affected by the R.chinensis Mill.In summary,it can be seen that S.chinensis and Rhus chinensis Mill genes related to growth and differentiation have appreciable correlation.It is necessary for us to further test our findings by adding more samples and data.