The Function Of Chorispora Bungeana CbF2H Gene

The low temperature affect plant distribution and cause crop yields to a significant reduction,so the study of plant cold response gene function and reaction mechanism is not only of theoretical significance,but also has huge economic benefits.At present,low temperature tolerance mechanisms of the model plant Arabidopsis have been studied extensively,but the plant with a strong cold resistance genetic background has been little studied.Chorispora bungeana Fisch.C.A.Mey is a typical perennial herb of alpine periglacial plant species.Chorispora bungeana can survive at frequently fluctuant and freezing temperatures.Therefore,Chorispora bungeana is ideal species for the study of plant freezing tolerance genes.This research is conducted with Chorispora bungeana as the experimental material,and CbF2H gene(up-regulated at-4?)as the object of study,which studies the relationship of CbF2H gene and Chorispora bungeana frost stress.The function of CbF2H gene is unknown,so the subject also studied Arabidopsis homologous genes AtF2H.The main results are as follows:1.The expression of CbF2H gene at transcription level was up-regulated under cold stress and salt stress treatment.Therefore,CbF2H gene may have a complex mechanism to response cold and salt stresses.2.Purified proteins and antibodies of CbF2H were obtained.By Western-blot it was revealed that the expression of CbF2H at translation level was first down-regulated under cold stress treatment.3.The subcellular localization of CbF2H was identified by transient expression of GFP in tobacco leaf cells and stable genetic expression of GFP in Arabidopsis plants.It was localized on cell membrane and nucleus.4.the results indicated that AtF2H was expressed during the whole process of its growth,and in different tissues of Arabidopsis thaliana,especially in the vascular tissue with greater expression.It is speculated that AtF2H may involve in nutrient transport in Arabidopsis.5.The phenotypic differences of Arabidopsis wild type and AtF2H T-DNA mutant were investigated.The results show that the lack of AtF2H gene can cause Arabidopsis to advance into the reproductive growth stage with the characteristics of a smaller number of rosette leaves,earlier bolting,earlier flowering,earlier fruiting and less seeds than wild type.6.The survival rate of AtF2H T-DNA mutant is lower than wild type under cold stress treatment.It is showed the lack of AtF2H gene makes Arabidopsis plants easier death.7.Physiological indicators of wild type and AtF2H T-DNA mutant were measured under cold stress treatment.It is manifested the lack of AtF2H gene reduces Arabidopsis plants freezing tolerance.In summary,CbF2H gene may involve in resistance to low temperature and salt stress pathway in Chorispora bungeana.AtF2H gene may change its growth process and plays an important role at the freezing tolerance of Arabidopsis.In a word,the function of both CbF2H gene and AtF2H gene was studied for the first time.It is preliminary that the function of low-temperature resistance of CbF2H was studied,which provides a theoretical basis for further research.