The Functional Research On CbCBF4

The transcription factor CBF4regulates the cold, drought and salt stress in the plant is the important member of CBF family. It can specifically bind to DRE/CRT, a cis-acting promoter element belongs to the COR gene, induced COR gene’s expression. Chorispora bungeana Fisch. et Mey is a Rare plant lives in the Alpine Region.Its transcription factor CbCBF4is answered in the cold stress. We used the technology of RACE, Western-blot, Double enzyme, Semi quantitative RT-PCR. Real-Time PCR and Transient expression of fluorescent fusion proteins in tobacco plants to study CbCBF4, of which expression levels is increasing when it under the cold stress. The conclusions are shown as follows:1. Cloned the gene of CbCBF4by the technology of RACE. Analyzed the protein’s structural characteristics of CbCBF4by the Bioinformatics software.2. Expressed the gene of CbCBF4in prokaryotic. Purified of protein with affinity chromatography. Lied the foundation for the next step on the level of translation regulation.3. Studied the change of CbCBF4gene expression under the low temperature stress by Semi quantitative RT-PCR. Revealed CbCBF4responsing to low temperature stress. Showed the difference in the level of the transcriptional with the reduction of the temperature.4. Studied the change of CbCBF4gene expression under high salinity stress by Real time PCR.Showed CbCBF4had a rise trend at the later stage of the high salinity stress.5. Based on the results of Subcellular localization, CbCBF4was localized on cell membrane.In conclusion, the paper has not only provided academic basis in digging resistance gene resources, but also reveals CbCBF4playing an important role in the cold and high salinity stress. CbCBF4is related to the maintenance of membrane structure stability and mobility. So it has important significance in researching the resistance mechanism of Cb.