| 1 ObjectiveTo observe the effects of hypoxic exercise on the changes of related protein in insulin signal transduction pathway in skeletal muscle of diabetic rats,and to explore the possible mechanism of hypoxic exercise on diabetes treatment.2 Method10 were randomly selected from 60 SD rats aged 6 to 8 weeks as normalcontrol group.The rest 50 SD rats were fed with high fat diet 4 weeks,and injected intraperitoneally with STZ once to induce diabetes model.To establish the model,fasting blood glucose of rats should be not less than 16.7 mmol/L.After modeling success,30 diabetic rats were divided randomly into 3 groups,hypoxic exercise group,normoxic exercise group,diabetic group,10 rats ineach group.Hypoxic tent system was used to simulate hypoxic environment of 2500 m latitude with oxygen concentration at 15%.After 3-day’s adaptive train-ing on treadmill,the exercise groups stared normal training,and were trained 1h per day,6 days in a week for 4 weeks.After the last exercise,the rats w-ere fasted for 12 hours.Then after pentobarbital anesthesia by intraperitoneal injection blood was taken from abdominal aorta and rats quadriceps were disse-cted.And the insulin in blood,GLUT4,AMPK,PI3 K.PKB in quadriceps wer-e measured.All the data were analyzed by using one-way ANOVA on SPSS19.0 software and results were showed with mean ± standard deviation.3 Results(1)Compared with normal control group,GLUT4 of hypoxic exercise group was decreased(P <0.05);GLUT4 of normoxic exercise group was decreased si-gnificantly(P<0.01).Compared with diabetic group,GLUT4 of normoxic exer-cise group was increased(P<0.05);GLUT4 of hypoxic exercise was increased significantly(P<0.01).Compared with hypoxic exercise group,GLUT4 of norm-oxic exercise group was a little lower,and had lit tle difference.(2)Compared with normal control group,PI3 K of hypoxic exercise group and normoxic were decreased significantly(P <0.01).Compared with diabetic group,PI3 K of hypoxic exercise group and normoxic exercise group were incr-eased(P<0.01).Compared with hypoxic exercise group,PI3 K of normoxic exer-cise group was increased(P<0.05).(3)Compared with normal control group,PKB of hypoxic exercise group and normoxic were decreased significantly(P <0.01).Compared with diabetic group,PKB of hypoxic exercise group and normoxic exercise group were increased(P<0.01).Compared with hypoxic exercise group,PKB of normoxic exercise gr-oup was increased(P<0.05).(4)Compared with normal control group,AMPK of hypoxic exercise group was decreased(P <0.05);AMPK of normoxic exercise group was decreased si-gnificantly(P<0.01).Compared with diabetic group,AMPK of hypoxic exercisegroup and normoxic exercise group were increased(P<0.01).Compared with hypoxic exercise group,AMPK of normoxic exercise group was decreased(P<0.05).(5)Compared with normal control group,insulin of hypoxic exercise group and normoxic were decreased significantly(P <0.01).Compared with diabetic group,insulin of normoxic exercise group was increased(P<0.05);insulin of hypoxic exercise was increased significantly(P<0.01).Compared with hypoxic exercise group,insulin of normoxic exercise group was decreased significantly(P<0.01).4 Conclusions:Normoxic exercise could improve the activity of AMPK,PKB and PI3 K,and increase glucose transporter protein and insulin.Under hypoxic exercise,the activity of AMPK,PKB and PI3 K in diabetic rats could be improved and glucose transporter protein and insulin could be increased significantly.Effects of hypoxic exercise on AMPK,PKB,PI3K and GLUT4 in skeletal muscle of diabetic rats during insulin transduction are more significant. |
