A Rat Model Of Meridian Patency And Its Effect On Metabolism Were Established By Ligation

ObjectiveAbnormality of qi and bloodis one of the pathogeneses of Zang-Fu organs and meri diandisorders.Meridian blockage is one of the main pathological change resulting in a bnormality of qi and blood.There are fewer mature animal pathological models ofmeri dian blockage and less research on them.In this study,based on thelow hydraulic resi stancechannel(LHRC)theory,pathological meridian blockage model in rats was establis hed by ligation.Through the measurement of dietary intake,urine and fece in model ra ts,the effects of the model on rat metabolism were observed to assess the effectivene ss of the model.We hope to provide an effective experimental animal model for futur e meridian research.Method1.Experiment 1 Establishment of pathological meridian blockage model in rats by ligation:48 male SD rats were randomly divided into 4 groups(n=12).The Ren meridian low electricresistance line(LERL)was measured first.Ligation group 1 ligated 1 point on the Ren meridian LERL;ligation group 2 ligated 2 point on the Ren meridian LERL;ligation group 3 ligated 3 point on the Ren meridian LERL;Ligation group 4 ligated 1 point out of the Ren meridian LERL,the ligation width was 1cm,and the ligation interval was 1-2mm.The interstitial fluid pressure wave propagation method was used to measure the amplitude of the interstitial fluid pressure wave propagation of the groups before and after the ligation.2.Experiment 2 The effect of pathological meridian blockage model on metabolism:The experiment was carried out in batches,a total of27 male SD rats were selected and divided into blank control group(control group),Stomach meridian blocking group(SMBG)and Kidney meridian blocking group(KMBG),with 9 rats in each group.On the blocking meridian day,ligation was used to block theLERL along stomach meridian to SMBG and kidney meridian to KMBG.The rats of control group were anaesthetized only.Observe the daily urine volume,weight of wet and dry feces,drinking water volume and food intakefor three consecutive days in the week before modeling and the sixth week after modeling,and calculatethe water content of fece.3.Experiment 3 Comparison of metabolic status of rats inmetabolicmonitoring cages of Comprehensive Lab Animal Monitoring System and ordinary rat cages:In the study of 6 ratsin the last batch of experiment 2,the changes of drinking watervolume and food intake in rats ofblank control group(n=2),SMBG(n=2)and KMBG(n=2)were observed during three consecutive days in ordinary rat cages(50cm×33cm×20cm)and metabolicmonitoring cages(32cm×20cm×18cm).Result1.Experiment 1:The change rate of interstitial fluid pressure wave before and after ligation was 8.17%,-7.83%,-13.27%and-52.80%respectively in four groups.There was no significant difference between the ligation group 4,the ligation group 1 and the ligation group 2 after ligation,but the change rate of the three groups increased from positive to negative.The amplitude of the ligation group 3 was significantly reduced(P<0.01)after ligation compared to before ligation.2.Experiment 2:2.1 Urine volume:The urine volumeof control group in the sixth week after modeling increased by 28.12%compared with the week before modeling(P<0.05);the urine volume of SMBG in the sixth week after modeling increased by 59.28%compared with the week before modeling(P<0.05);the urine volume of KMBGin the sixth week after modeling increased by 41.33%compared with the week before modeling(P>0.05).The change rate of urine volume between the week before modeling and the sixth week after modelingwas not statistically significant among the groups(P>0.05).2.2Water content of feces:The mean values of the water content of feces of rats in control group,SMBG and KMBG was 44.71%,40.87%and 43.39%respectively in the week before modeling.The mean values of the water content of feces in the sixth week after modeling in three groupswere 43.88%,40.26%and 42.27%respectively.There were no statistically significant changes in the three groups(P>0.05).The change rate of water content of feces between the week before modeling and the sixth week after modelingwas not statistically significant among the groups(P>0.05).2.3Drinking water volume:The drinking water volumeof control group in the sixth week after modeling decreased by 12.99%compared with the week before modeling(P<0.05);the drinking water volume of SMBG in the sixth week after modeling decreased by 2.71%compared with the week before modeling(P>0.05);the drinking water volume of KMBGin the sixth week after modeling decreased by 8.58%compared with the week before modeling(P>0.05).The change rate of drinking water volume between the week before modeling and the sixth week after modelingwas not statistically significant among the groups(P>0.05).2.4 Food intake:The food intakeof control group in the sixth week after modeling increased by 5.64%compared with the week before modeling(P>0.05);the food intake of SMBG in the sixth week after modeling decreased by 2.02%compared with the week before modeling(P>0.05);the food intake of KMBGin the sixth week after modeling decreased by 2.03%compared with the week before modeling(P>0.05).The change rate of food intake between the week before modeling and the sixth week after modelingwas not statistically significant among the groups(P>0.05).Conclusion1.The rat pathological modelofineridian blockage was sucessfully established by ligation,and thereliabilityand stability of the model was proved by tissue hydraulic wave propagationmethod.It can make the pathological meridian blockage modeling impossible in the most commonly used experimental animal rats,laying a new model organism basis for the future research on acupuncture and meridians.2.Sixth weeks after modeling,the urine volume in the blank control group and the SMBG was significantly increased,and the urine volume in the SMBG was more than 1 times higher than that in the blank control group,while theurine volume in the KMBG did not increase significantly.In the blank control group,the drinking watervolume decreased,and the food intake increased.However,the drinking watervolume in the SMBG and the KMBG decreased less and the food intake increased less.It shows that blocking the stomach meridian can cause a significant increase in urine volume in rats,and there is a positive trend of Excess Syndrome of Yangming Fu-Organs.It shows the trend ofthe influence ofligating the stomach meridianon the metabolism of stomach-associated organs.Ligation of the kidney meridian can affect the fluid metabolism in rats,showing no significant increase in urine volume,suggesting that blocking the kidney meridian may affect the metabolism of kidney-associated organs.3.Due to the small size of metabolic monitoring cages,it may have a certain impact on the metabolism of rats,resulting in a decrease in their drinking watervolume and food intake,suggesting that the future study of metabolism in rats should be conducted in larger cages.