The Method Of Diarrhea In The Treatment Of Multiple Sclerosis And The Effect Of Diarrhea Into The Distribution Of Myelin Repair

PurposeMultiple sclerosis(MS)is an immuno-mediated central nervous system(CNS)inflammatory demyelinating disease.The course of repeated onset and high disability rate lead to poor prognosis.The purpose of this study includes two parts,the first part,by the yong-ping fan,a professor at the diagnosis and treatment of the TCM clinical data of 112 patients with MS data mining,discusses the experience and MS syndrome distribution regularity for use,etc.,so as to enrich the theory of syndrome differentiation of traditional Chinese medicine treatment of MS,guide the clinical and experimental research,at the same time provide the clinical basis for the second part studies the content of.The second part is by observing the effective component of capsule,invigorating the kidney and marrow catalpa alcohol allergy,emodin on experimental encephalomyelitis(experimental autoimmune encephalomyelitis,EAE)in the brains of mice oligodendrocyte transcription factor(oligodendrocyte transcription factor,Olig)1 and Olig2 prevented expression to explore the effects of Bushen Yisui Capsule purging compatibility supplement in the mechanism of action of myelin repair.MethodsThe first part is to select MS patients who were admitted to Beijing tiantan hospital affiliated to capital medical university from December 2010 to December 2017.Meet eligibility criteria,a total of 112 cases were done by professor yong-ping fan of syndrome differentiation and prescription,using frequency analysis method to the patients' general information,clinical symptoms,the law of syndrome classification,medication were analyzed,and the zang-fu organs is deficient in MS and wind phlegm damp heat and blood stasis syndrome elements as well as the significance of treatment for MS yong-ping fan professor xie medicine regularly.In the second part,80 C57BL/6 mice were randomly divided into the normal group,the model group,the hormone group and the compatibility group(40mg/kg of catalpol,1mg/kg of emodin),20 mice in each group.The model group,the hormone group and the compatible group were induced to produce EAE by subcutaneous injection of myelin oligodendrocyte glucoprotein 35-55(mog35-55)polypeptide.Materials were collected on day 18(peak of symptoms)and day 40(remission of symptoms)after immunization induction.Transmission electron microscoe(TEM)was used to observe the loss of myelin sheath,and western blot(WB)was used to observe the expression of Olig1 and Olig2 proteins.Real-time fluorescent quantitative reverse transcription polymerase chain reaction(real-time reverse transcription-polymerase chain reaction,real-time rt-pcr)observation Olig1 and Olig2 prevented gene expression.ResultAmong 112 MS patients,kidney deficiency(hepatic and kidney Yin deficiency)was the most commonly recognized type of TCM dialectics,with 72 cases in total,including 20 cases with simple kidney deficiency and 52 cases with hepatic Yin deficiency.There were 31 cases of qi deficiency.A total of 105 out of 112 patients had stasis syndrome differentiation.A total of 72 cases were diagnosed with phlegm.A total of 71 cases were diagnosed with phlegm stasis.The pathogenesis of MS is based on kidney deficiency.Phlegm,blood stasis,dampness,heat and internal wind are the standard factors.When the patient come to see doctor the top three kinds of symptoms including mainland disorder,limb numbness,limb weakness,before the first symptoms appear in the frequency of the highest three symptoms are numbness,muscle weakness,vision loss treatment including tonic drugs(qi and blood,Yin and Yang)accounted for 23.0%,xie real evil class(invigorate the circulation of medicine,clear heat,flat extinguish wind medicine liver and nourish the medicine(indications),spleen,eliminating phlegm,medicine,water moisture penetration,begin to understand medicine,diarrhea medicine,chemical medicine,xiaoshi drugs)accounted for 72.1%,other(sedative medicine,astringent and hemostatic),accounted for 4.9%.Catalpol with emodin can significantly reduce EAE mice myelin damage,reduce peak symptoms and symptoms remission nerve function score,TEM showed compatibility groups of myelin sheath the ring of layered structure compared with model group mice break loose can alleviate,WB results show that the compatibility group 40 days Olig2 prevented protein expression in the model group increased(P<0.05),see Oligl protein expression was not significant difference(P>0.05).Real-time rt-pcr results showed that the expression of Oligl and Olig2 genes was higher in the combination group on day 18 and day 40 than in the model group(P<0.05).Conclusion112 MS patients in traditional Chinese medicine dialectical to viscera deficiency of kidney,liver and kidney Yin deficiency)is given priority to the most,is present throughout the course of MS factors,phlegm and blood stasis and phlegm and blood stasis and see most,fill treatment in accordance with diarrhea.The combination of tonifying and relieving compounds in Bushen Yisui Capsule improving the expression of Oligl and Olig2 in the brain of EAE mice can alleviate the damage of EAE myelin sheath and promote the regeneration of myelin sheath to a certain extent.