Deficient Expression Of The Transcription Factor Sp3 In Peripheral Blood Mononuclear Cells In Multiple Sclerosis Patients And Exploration Of Its Influencing Factors

Background Multiple sclerosis(MS) is a cause unclear autoimmune inflammatory demyelinating. disease of central nervous system. Increasing evidences show that both genetics and environmental factors involved. The susceptible gene of MS is one of the research hot spots. MS is an autoimmune disease, so the immunogenetic study is mainly focused on Major Histo-compatibility Complex(MHC), T cell receptor(TCR), immunoglobulin heavy chain variable gene(lgGHV), myelin basic protein(MBP) which is the putative antigen of MS, etc. So far, no encouraging results were found in addition to weak linkage exiting in MHC in recent 100 years. To find the susceptible gene of MS will help to understand the genetic role in MS pathogenesis. The ideal objective for MS genetic study is twin.Differential display PCR(DDPCR) is a new technique which is suitable to find the different gene expression between the individuals with same genetics background. Grekova at al. used this technique to detect a pair of homozygote twin with MS patients (one with MS, and another without). Several different genes expressed in two twin were found, and one among them is Sp3. Sp3 protein modulates the expression level of many gene in human as a transcriptional factor. Sp3 can exert different role to different genes, including inhibit and activate the transcription in the level of RNA. Sp3 is found after Sp1 which is first found can bound to the Simian Virus early promoter and can induced its transcription. Sp3 can bind with GC/GT box, both of which exist ubiquitously in human. And just this determines the important role that Sp3 to...