| Objective: Aging is a major risk factor for most chronic diseases. Over the past century,social and economic development has made significant progress in medical and health care.Human life has been steadily increasing, leading to a dramatic increase in the world's elderly population, and aging-induced liver morphology and molecules Learning changes that affect the liver function of elderly patients, affect the liver drug metabolism, increase the risk of various liver diseases, as a poor prognostic factor, leading to increased mortality, therefore,more understanding of the molecular mechanism of aging, Treatment strategies that can prevent aging-induced liver disease progression are particularly important. The rat model of aging was induced by inhalation of ozone, and the liver injury in aged rats and the protective effect of Chinese herbal compound hope solution on liver injury in aged rats were investigated by biochemical markers, pathological sections and proteomics.Methods: Forty-eight male SD rats were randomly divided into 6 groups (n = 8). (1.2 ±0.2 ppm) 10h / d × 45d, in which the model group was fed with drinking water daily, and each group was treated with ozone (1.2 ±0.2 ppm) for 10h / d × 45d, Simultaneous tonic vitamin E(13.5mg / kg) and hope I liquid (45,90,180 mg / kg) treatment. Modeling 45 days drawn, take the rat liver. The contents of malondialdehyde (MDA), superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) and catalase (CAT) in liver tissue were detected. HE staining was used to observe the morphological changes Proteomics methods were used to detect differential proteins and to correlate proteins with significant differences in biological processes, molecular functions, cellular components and biological signaling pathways.Results: The contents of CAT, SOD and GSH-Px in the model group were significantly higher than those in the control group (P <0.01). The number of hepatocytes decreased, the cell volume and the nucleus were compensated The liver cells were stained with chromatin in the cytoplasm of hepatocytes, and the chromatin condensation of hepatocytes was significantly different from that of control, function and regulation. The results showed that there was significant difference between the control group and the control group Group, the difference was significant in the model group, and the differential protein was significantly down-regulated. (P <0.01). The contents of CAT, SOD and GSH-Px were increased (P <0.01).The histological changes were observed in fat-like degeneration, the cell arrangement was dense and the volume of hepatic sinusoids increased , The arrangement of liver cells and the accumulation of lymphocytes were significantly higher than those of the model group, and the difference was significant in 140 groups, and 140 differential proteins were significantly down-regulated, which were related to the structure, metabolism, function and regulation of liver Protein marked callback.Conclusion: Ozone can damage the structure of liver in rats and induce the change of multichannel protein, which may affect the expression of SOD, GSH-Px and CAT and the increase of MDA content in liver injury induced by inhalation of ozone , Repair the liver tissue structure and morphological changes, and by regulating the liver tissue structure,function, metabolism and other related channels, to prevent oxidative stress, the establishment of the new environment within the environment, for ozone-induced aging of liver damage effect. |
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