| Purpose:This research adopts the high-fat feeding method,phlegm turbidity pixu tanzhuo model is established for a long time,through the observation Xiangsha Liujun Pills effect on gastric mucosal cells JAK2 / STAT3 pathway,explore the invisible material foundation of phlegm and spleen phlegm method of biological mechanisms,as well as control the obese people suffering from gastrointestinal disease risk provides new train of thought.Material and method: 1.Group 40 SPF male SD rats,were randomly divided into control group,the phlegm turbidity pixu tanzhuo group(model group),Xiangsha Liujun Pills lower dose treatment groups(LDMEL),Xiangsha Liujun Pills median dose treatment groups(MDMTX)and Xiangsha Liujun Pills high dose treatment groups(HD)2.Pixu tanzhuo copy methods and the evaluation standard of long-term high fat feed method.Evaluation criteria: obesity,eat less,lazy to move,and dyslipidemia.Quality evaluation method: electronic scale measurement on an empty stomach,Kuang field experiment testing within 5 min movement distance of rats;Metabolic cages record 24 h food intake;Caudal artery blood testing serum triglyceride(TG)level 3.Therapeutic Method After Xiangsha Liujun Pills sand powder,XLG,XMG,XHG were 112.5 mg/kg,225 mg/kg and 550 mg/kg 2 times irrigation in the morning and evening,With distilled water irrigation,obesity in the control group,model group,the volume and drug is the same volume,30 d.4.Blood fat levels Antuomatic biochemical analyzer was used to check serum lipid profile,TG,TC,HDL,LDL 5.Serum oxidative stress and endotoxin levels ELISA was chosen to check serum levels or activity of SOD,MDA,GSH-Px and LPS;6.The gastrointestinal mucosa cell JAK2 / STAT3 / Bcl-2 Immunohistochemistry wasemployed to detect expressions of p-JAK2 and STAT3,Bcl-2 in gastric mucosa,Small intestinal mucosa and colon mucosa 7.Data Processing Using SPSS17.0 statistical software for processing,the data to X_±SD said,mainly USES the comparison between groups,with P < 0.05 for statistical significance.Results: 1.Blood fat levels: compared with the control group,TG,TC and LDL levels of the model group significantly increased(P < 0.01,P < 0.05,P < 0.01).HDL levels respectively in the model group,XLG group and XMG group sharply declineed(P < 0.01).Compared with the model group,TG and LDL levels of XLG,XMG and XHG groups significantly decreased(all P < 0.01),but HDL levels of the XHG group increased obviously(P < 0.01).2.Serum oxidative stress and endotoxin levels : SOD levels in the model group were significantly lower than the control group(P < 0.05),and in the XLG,XMG and XHG were obviously higher than that of the model group(P < 0.05,P < 0.01,P < 0.01).GSH-Px activity in the model group,XLG group and XHG were significantly lower than the control group(all p < 0.01),meanwhile XLG,XMG and XHG were all higher than the model group(p < 0.05,p < 0.01,p < 0.01).MDA level in the model group and XLG group was significantly higher than that of the control group(P < 0.01),and that in XMG group and XHG group war obviously lower than the model group(P < 0.01).At the same time,MDA level in the XMG group and XHG was significantly lower than the XLG group(P < 0.05,P < 0.01).LPS level in the model group and in the control group both significantly rise(P < 0.01),and that in the XLG,XMG and XHG groups were all lower than in the model group(all P < 0.01).3.Immunohistochemical method to detect JAK2 / STAT3,Bcl-2 expression of rat gastric mucosa: Compared with the control group,JAK2 expression of rat gastric mucosa in the model group significantly increased(P < 0.01),and JAK2 expression of rat gastric mucosa in the XMG and XHG groups obviously decreased(both P < 0.01).Compared with the control group,STAT3 expression of rat gastric mucosa in the model group significantly increased(P < 0.01).Meanwhile,STAT3 expression in the XMG group and XHG significantly increasedcompared with the model group(all P < 0.01).Compared with the control group,Bcl-2 expression of rats gastric mucosa in the model significantly increased(P < 0.01),and Bcl-2 expression of rats gastric mucosa in the XLG,XMG and XHG groups decreased significantly compared with the model group(P < 0.05,P < 0.01,P < 0.01).4.Immunohistochemical method to detect JAK2 / STAT3,Bcl-2 expression of rats small intestine mucosa: JAK2 and STAT3 expression of in the model group was significantly higher than the control group(P < 0.01,P < 0.05).JAK2 expression in the XLG,XMG and XHG groups was lower than the model group(P < 0.01,P < 0.01,P < 0.01).STAT3 expression in the model group was obviously higher than that of the control group(P < 0.05).STAT3 expression was significantly lower than the model group(P < 0.05).Bcl-2 expression in the XLG,XMG and XHG groups was significantly lower than in the control group.Compared with the XLG and XMG groups,Bcl-2 expression in the XHG group obviously increased(P < 0.01,P < 0.01).5.Immunohistochemical method to detect JAK2 / STAT3,Bcl-2 expression of rat colonic mucosa: JAK2,STAT3 and BAX expression in the model group were significant higher than the control group,and Bc1-2 expression was obviously lower than the control group(P < 0.01).Conclusion: 1.The invisible material foundation of phlegm and blood lipid abnormalities,excessive oxidative stress and endotoxin release and so on 2.Spleen phlegm method of biological mechanisms including the regulation of blood lipids,against oxidative stress,inhibit excessive release of endotoxin,intervene in the gastrointestinal mucosa cells JAK2/STAT3/Bcl-2.3.Spleen phlegm may help reduce the risk of obesity crowd associated gastrointestinal disease... |
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