Experimental Study On The Effect Of Xintongtai On The Stability Of Atherosclerotic Plaque In ApoE -/- Mice

Objective: Discussion the mechanism of the Xintongtai influence Plaque stabilityin ApoE^-/-mice AS.Methods: 42 6 to 8-week-old male mice ApoE^-/-（C57BL/6J）.Housed in SPF grade schoollaboratory.high-fatdiet（2%Cholesterol+10%lard +10%custard powder +0.2%Pig bile salt +77.8%Ordinary）feed,another 12 C57BL/6J wild mice as a group only,payable-to an ordinary diet.12 weeks later,out ofthe two mice in the random 2,HE stained observation,obviously shows ApoE^-/-mouse unstable atherosclerotic plaque formation and C57BL/6J in mouse aorta smooth,showsthat AS model of success.ApoE^-/-mice by random number table were randomly dividedinto 4 groups:atorvastatin calcium-group,but Xintongtai low-dose group,but Xintongtai high dosegroups plus controls（C57BL/6J in mice）.Gavage for 8 weeks,animals were treated under sterile conditions,after HE staining,aortic pathologic changes（plaque area/arterial wall cross-sectional area（%））under high power microscope,observe the Changes of collagen content inplaque（collagen content/plaque area（%））under high power microscope too,use Enzyme method and Colorimetry to test Blood lipid index,ELISA test CRP 、 MMP-9 、 TIMP-1 in Serum and MMP-9 、 TIMP-1expression detected by IHC.Results: 1.Compared with the group,Drug group were significantly reduce TC,TG,LDL-c and rise HDL-C,and High-dose group of xintongtaiare better than low-dose group（P < 0.01）,but high dose groups no differences between the statin group（P >0.05）.2.Compared with the group,Drug group were significantly reduce CR 、 MMP-9 、TIMP-1 in Serum,and High-dose group of xintongtaiare better than low-dose group（P<0.01）,but high dose groups no differences between the statin group（P>0.05）.3.Compared with the group,Drug group were significantly inhibited positive expression of MMP-9 and promoted TIMP-1 in plaque,and High-dose group of xintongtaiare better than low-dose group（P < 0.01）,but high dose groups no differences between the statin group（P>0.05）.Conclusion:1.Xintongtai granules canbesignificantlylowered AStotalserumcholesterol（TC）,triglyceride（TG）,low-density lipoprotein cholesterol（LDL-c）andraise high-density lipoprotein cholesterol（HDL-c）,those which explain Xintongtai can intervene the AS development and stable the plague through regulating the blood lipids.2.Microscopic observations show,xintongtai-can improve AS pathological changes i n mice aorta and reduce Plaque area ratio,and high dose groups were better the low dose groups.3.Xintongtai granules can be stable plaque throught collagen content.4.Xintongtaimay be reduced systemic inflammation to delay the occurrence and development of AS to stabilize plaque,because of,its can be reduced CRP、MMP-9 、TIMP-1 in Serum,and High-dose group of xintongtaiare better than low-dose group.5.Xintongtai granules played a role in stabilizing atherosclerotic plaque by interfering with the degradation of ECM,through inhibited positive expression of MMP-9,promoted TIMP-1 in plaque and regulated balance of MMP-9/TIMP-1.