Clinical Observation Of Gengnian Decoction On Patients With Menopausal Syndrome And Related Factors Of Ovarian Granulosa Cell Apoptosis

Objective:To evaluate the effect of gengnian decoction on sex hormones and menopause syndrome patients for menopausal transition model with kidney Yang deficiency rats uterus ovarian index, ovarian tissue morphology, ovarian granulosa cell apoptosis factors bax, baxmRNA, Bel-2, the influence of the bcl-2 mrna, and to explore its action mechanism.Methods:1.We choose 60 cases of patients with menopause syndrome, clinical manifestations of the kidney Yang deficiency syndrome type or kidney Yin and Yang are two virtual type, were randomly divided into treatment group and control group, respectively with soup and material treatment to more. Before and after treatment determination of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E2) changes.2.We choose natural aging perimenopausal female SD rats, coxal muscle around alternating muscle hydrocortisone injection construction of kidney Yang deficiency type perimenopausal rats model, the model rats were andomly divided into soup group, teaching materials, levy more years love group and saline group; An alternate young group, elderly group SD female rats as the control group, to intramuscular equal capacity of saline solution.To lavage them by different drugs for 15days,and gnaesthetize them after 24hours at the last time gavage.put the femoral arterial blood executed, profile control the rats'uterus and ovaries. Count the model rat uterine ovarian index, observe the model rat uterus, ovarian tissue factor, ovarian tissue morphology and ovarian granulosa cell apoptosis Bax, BaxmRNA, Bcl-2,the change of the Bcl-2 mrna.Results:The treatment group patients follicle-stimulating hormone (FSH) and luteinizing hormone (LH) generated decreased before the treatment, But it didn't have any significance in statistics (p> 0.05),estradiol (E2) was higher than before the treatment and had statistical meaning (p< 0.05), the treatment group is better than the control group.2,Vaginal epithelial cells mature index:compared with the more years soup group compared young group, the elderly group and the saline group, the more years soup group had statistical significance (p< 0.05), but didn't have obvious difference compared with the textbooks group, and levy love group (p> 0.05), uterine and ovarian index of more years soup group had statistical significance (p< 0.05). Ovarian tissue pathological slices, from three aspects of follicular, blocking, good expansion, more years soup group had statistical significance or obvious statistical significance (p< 0.05 or p< 0.01). Gengnian decoction for bax, baxmRNA, bcl-2, the bcl-2 mRNA had statistical significance or obvious statistical significance (p<0.05 or p<0.01).Conclusion:Gengnian decoction can increased the estrogen levels, add the vaginal epithelial cells maturity, uterus and ovarian index, promote the follicular development, control the follicular atresia and expansion, increase ovarian granulosa cell apoptosis factor the Bcl-2, the Bcl-2 mrna level and control the apoptosis factors Bax, BaxmRNA positive expression.