Based On The Theory Of "spleen And Protect The Heart", The Mechanism Of The Intervention Of Jianpi Recipe On Atherosclerosis Was Discussed

Purpose:Based on the theories of "strengthening the spleen and protecting the heart",the aim of the study is to discuss the mechanism of the early intervention of invigorating the spleen on atherosclerosis Apo E-/-mice,to provide experimental results for early intervention of atherosclerosis.Material and method : Fifty 9-week-old male mice are randomly divided into 5groups:the model group,the western medicine group,Jianpifang- the low dose group(J-L),Jianpifang- the medium dose group(J-M),Jianpifang- the high dose group(J-H). And another ten male C57BL/6N mice from the same strain as the normal group are prepared to be compared with the mice from five groups. The mice from comparative group are fed with general diet,while the ones from five test groups are all given high-fat diet.Simultaneously,the mice of the normal group and the model group are given normal saline by gavage once a day for 12 weeks.Three Jianpifang groups are respectively given Jianpifang through different dose lavage once a day for 12 weeks. The ones of western medicine group are given the solution of Atorvastatin Calcium Tablets once a day for 12 weeks. From the13 th week,HE staining is adopted to observe the Apo E-/-mice aorta morphological changes.The ELISA is done to measure the aortic tissue serotonin(5-HT),5- HIAA(5-HIAA), interleukin-6(IL-6) and tumor necrosis factor(TNF-?) content changes.The BL-2000 digital image analysis system is made for the pathology gray value measurement,testing serotonin expression 2A receptor(5-HT2AR),IL-6,TNF-?.Results:1. The demonstration of the aortic sections of the mice under the microscope:The normal group:The artery structure is clear, smooth.The endothelial cells are intact.The elastic membrane and the elastic fibers are with the normal shape and the smooth muscle cells without any obvious pathological changes;The model group: The smooth muscle cells are in disorder arrangement with the obvious thickening intima,the unsmooth surface,elastic fiber breakage,visible endothelial cell lipid infiltration into the lumen,and a large number of hyperplastic inflammatory cells,lipid stripes and plaque formation;The western medicine group:The artery structure is clear,with the smooth inner membrane,the regular endothelial cell,the regular arrangement of the smooth muscle cell,the complete elastic fiber,the small amount of proliferation of inflammatory cells;The J-L group:The aortic structure is not clear,with the irregular endothelial cells,the disorder arrangement of the smooth muscle cells,elastic fiber breakage,and the visible aggregation of small amounts of infiltration of inflammatory cells;The J-M group: Aortic intima is smooth,with a small number of endothelial cells,the more regular arrangement of the smooth muscle cells,and the decreased inflammatory cells;The J-H group:The overall structure of the aorta is clear,with the smooth inner membrane,elastic fiber, the better endothelial cell repair,the more regular arrangement of the smooth muscle cells and even the obvious inflammatory cells.2. The detection of 5-HT, 5-HIAA, IL-6, TNF- alpha in aortic tissue.Compared with the normal group, the content of 5-HT,5-HIAA,IL-6,TNF-? from the model group increased(P<0.05)and compared with model group, those of each drug group decreased(P<0.05). The order of the intervention effect from high to low respectively shows:The J-H group, The J-M group, the western medicine group, The J-L group.3. The results of 5-HT2 AR, IL-6, TNF-? in the aortic tissue of Apo E-/- miceThe gray value is inversely proportional to the positive expression.The more the positive expression and the darker staining are,the lower the gray value is. Compared with the normal group,the positive expression of 5-HT2 AR,IL-6,TNF-? from the normal group is up-regulated(P<0.05),while compared with the model group, the positive expression of those in each group is decreased( P < 0.05).The order of the intervention effect from high to low respectively shows:The J-H group,The J-M group,the western medicine group, The J-L group.Conclusion:1.The atherosclerosis model is able to be successfully reproduced for Apo E-/- mice fed with high fat diet for 12 weeks.2. Different concentrations of Jianpifang are able to improve the aortic structure of As mice,in which the J-H group is the most significant effect.3. Different concentrations of Jianpifang are able to regulate the content of 5-HT,5-HIAA,to reduce the expression of inflammatory factor IL-6,TNF-?, thereby to interfere with the formation of As by means of 5-HT2 AR,in which the J-H group is the most significant effect.