| Objective: Correlation between touch cable acetic acid glacial ice Rhizoma Anemarrhenae was established by intragastric infusion of cold syndrome rat model with gastriculcer and the proper conditions,were screened out to make cold syndrome rat model with gastriculcer was the best method to create an animal model;observation of Alpinia galanga,galangal,Alpinia katsumadai Hayata,Galanga,educational five kinds of Alpinia genus in cold syndrome gastriculcer rats for signs,morphology of gastric mucosa,blood rheology changes,the effects of cyclicnucle otide levels and their genetic relationship and the effectiveness of the common pharmacological effect of induction,interpretation of "relationship-drug-efficacy of traditional Chinese medicine and the pharmacolo gical effects to the same phylogenetic relationship of traditional Chinese medicine effect common seeking scientific basis.Methods: Set five glacial acetic acid group,the concentrations were5%,10%,15%,20%,25%,respectively,plus 4 DEGC Zhimu Decocti on making cold syndrome rat model with gastric ulcer,gastric ulcers,the ulcer index,pathological changes,natural restoration and animal deat hs,to determine the best ice acetic acid gastric perfusion concentration.After the determination of the optimum model concentration,the blank group,the model group,the 2 positive control group and the 5 group,the high and low dose group,10 rats in each group were set up.Except the blank group,other groups using ultimately determine the concen tration of acetic acid and modeling and 4 DEG C Zhimu Decoction in of,after the successful modeling,blank group,the model group was or ally distilled water,two positive control group were given service Fuzili zhong pills and cimetidine and other groups were given the drug.After4 days of administration to observe the gastric ulcer cold syndrome rats behavior characterization of changes in food intake,weight,coat color,rectal temperature,stool,mental state);the naked eye observation of morphology of gastric mucosa,ulcer was measured length and width calculation of ulcer area and ulcer index;the blood rheometer was used to measure blood rheological study indexes(blood viscosity,whole blood reduced viscosity,plasma viscosity,fibrinogen,erythrocyte is pressed product,cell electrophoresis time,Casson viscosity,Casson yield stress);enzyme linked immunosorbent assay(ELISA)for the determination of gastric tissue,plasma,platelets,vascular smooth muscle camp,cGMP content and calculation the ratio of cAMP / cGMP.Adopt SPSS19.0 to carry on statistics processing,with P < 0.05 for have statistics meaning.Results: 1 Acetic acid with ice cold syndrome rat model of Zhimu irrigation conditions to establish gastric gastric ulcer.15% The ulcer area of the concentration of glacial acetic acid was large,and there was no death,and the natural recovery was slow.Therefore,glacial acetic acid irrigation gastric duplication cold syndrome rat model with gastric ulcer best modeling conditions: rats were given or ally 4 DEG C ice,Rhizoma Anemarrhenae,water decoction and 15% a cetic acid solution,each 1/d,8:00 gavage ice Rhizoma Anemarrhenae,18:00 gavage glacial acetic acid for 4 days.Indoor environment setting temperature 16 degrees,humidity 60%.2 Five Alpinia medicine for stomach ulcers cold syndrome rats pharmacodynamics research.2 Effect of 5 kinds of Chinese medicine Alpinia on gastriculcer of rats with cold study(1)Effect on gastric ulcer of rats with behavioral signs of cold(1)Effect on the body weight of gastric ulcer cold syndrome rats: and compared with model group,the body weight of rats in drug group were increased(P < 0.05 or P < 0.01).(2)The effect on food intake of gastriculcer of cold syndrome rats: compared with the model group,the drug group rats food intake increased.(3)Of gastric ulcer of cold syndrome rat hair color score value of influence:and compared with model group,drug group subjects,except the Alpinia galanga low dose group,rats hair color score decreased(P < 0.05 or P <0.01).(4)On gastric ulcer of cold syndrome in the rectal temperature effect: and compared with model group,drug groups,the rectal temperature of the mice were increased(P < 0.05 or P < 0.01).(5)Of gastric ulcer of cold syndrome in stool,mental state: drug group rats stool volume decreased,relatively dry,forming,mental state has been restored,action is more flexible;shapeless stool pulpy of rats in model group,slow.(2)Effect on gastric ulcer of rats with cold blood rheology(1)Low dose,low dose(10/s puzzle(30/s value: effect of cold on gastric ulcer in rat model of blood viscosity with the model group,the subjects drug group rats whole blood high shear rate(200 / s)as well as blood cut(40/s significantly decrease(P < 0.01 or P < 0.05).With the exception of Alpinia galanga low dose group,and the remaining subjects drug group of blood cut II value was significantly reduced(P < 0.01 or P < 0.05);in addition to the low dose group,and the remaining subjects drug group,the whole blood low shear value was significantly reduced(P < 0.01 or P < 0.05);with the exception of Alpinia officinarum Hance Alpinia officinarum Hance,the puzzle of the low dose group,and the remaining subjects drug group,the whole blood low shear(1 / s)values were significantly reduced(P < 0.01 or P < 0.05).(2)The gastric ulcer cold syndrome rats whole blood reduced viscosity effect:and compared with model group,rats in drug group high shear reduced viscosity values decreased(P < 0.05 or P < 0.05);groups of low shear reduced viscosity values did not differ significantly(P > 0.05);except for a low dose of Alpinia galanga and other experimental drug group cut reduction viscosity values were decreased(P < 0.05 or P < 0.05).(3)The gastric ulcer cold syndrome rat red blood cell hematocrit,effects of cell electrophoresis time: and compared with model group,subjects drug group big mouse red blood cell hematocrit,cell electrophoresis time decreased(P < 0.05 or P < 0.05).(4)On gastric ulcer of cold syndrome in Casson viscosity,the Casson yield stress effects: and compared with model group,drug group subjects,except the big galangal low dose,the puzzle of the low dose group and other experimental drug group rats Casson viscosity,the Casson yield stress decreased significantly(P < 0.05 or P < 0.05).(5)The gastric ulcer of cold syndrome in plasma viscosity and protein fiber raw effects: compared with the model group compared each rat plasma viscosity,fibrin original difference is not obvious,the difference was not statistically significant(P > 0.05).(3)Effect on gastric ulcer of rats with gastric mucosal morphology of cold Compared with model group,the ulcer area and ulcer index were significantly decreased(P < 0.01).(4)Effect on gastric ulcer of rats with cold c AMP and the content of c GMP(1)Gastric tissue contents of c AMP and c GMP: and compared with model group,drug group rat gastric tissue contents of camp were increased(P < 0.05 or P < 0.05);c GMP content was not significantly different,there was no statistical significance(P > 0.05);weeding,nutmeg,low dose,Alpinia galanga low dose,educational low dose group and other experimental drugsthe ratio of camp and c GMP increased significantly(P < 0.05 or P < 0.05).(2)Plasma c AMP and c GMP contents: and compared with model group,subjects rats in drug group of plasma c AMP contents increased significantly(P < 0.05 or P < 0.05);c GMP content was not significantly different,no statistical significance(P > 0.05);subjects drug group in the plasma of the rats in the ratio of camp and c GMP increased significantly(P < 0.05 or P <0.05).(3)Platelet c AMP,c GMP contents: compared with the model group compared except the puzzle in the low dose group,the subjects drug group rat platelet c AMP level increased significantly(P < 0.05 or P < 0.05),the ratio of c AMP / c GMP increased significantly(P < 0.05 or P < 0.05);c GMP content was not significantly different,no statistical significance(P > 0.05).(4)Vascular smooth muscle camp and c GMP contents: and compared with model group,drug group rats vascular smooth muscle c AMP content was significantly higher(P < 0.05 or P < 0.05),the ratio of c AMP / c GMP increased significantly(P < 0.05 or P < 0.05);c GMP content was not significantly different,no statistical significance(P > 0.05).Conclusion:(1)The optimal concentration of acetic acid gastric ulcer in rat model of replication with acetic acid was 15% with Rhizoma anemarrhenae.(2)Five kinds of Alpinia belongs to the traditional Chinese medicine can by improving gastric ulcer cold syndrome model rats behavior characterization,blood rheology study,gastric mucosal morphology and increased gastric tissue,plasma,platelets and vascular smooth muscle c AMP content and index,showing heat resistance,play of temperature Tongxu blood,warm stomach,dispersing cold effect,to achieve cold syndrome in the treatment of gastric ulcer.The research results show that the five kinds of Alpinia belongs to the traditional Chinese medicine have similar pharmacological effects,indicating the existence of genetic relationship of traditional Chinese medicine,between property effects and the pharmacological effects exist in close contact,the correlation between the interpretation of phylogenetic relationships among the same herbs its potency and effectiveness provides experimental basis. |
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