Preparation Of Functionalized Graphene-based MOFS Composites And Analysis Of Glucose Non-enzymatic Electrochemical Sensing

Diabetes is a metabolic disorder syndrome that can cause death and disability and affect the life and health of human beings all over the world.Strict monitoring of blood sugar level plays a very important role in the diagnosis and management of diabetes.Natural enzymes have attracted much attention because of their high catalytic efficiency,high specificity,and diversity.As a biocatalyst,natural enzymes are liable to lose their activity due to the temperature and acid-base change.Consequently,researchers begin to pay attention to the related aspects of mimetic enzymes.Because of high specific surface area,chemical modification,controllable pore structure and simple functionalization,MOFs materials have broad application in the fields of separation,catalysis,sensors and biomedicine.In this work,MOFs materials and functional graphene were combined,and a high sensitivity for glucose was achieved by constructing colorimetric sensor and non enzymatic glucose electrochemical sensor.(1)NH2-MIL-88B(Fe),NH2-MIL-88B(Fe)derivatives were prepared by solvothermal method and NH2-MIL-88B(Fe)was combined with graphene oxide and magnetic graphene to obtain a higher activity of mimic peroxidase.H2O2 and glucose were detected by the high analogue peroxidase activity by using the composites as colorimetric probe.(2)Fe3O4@RGO with the best electrochemical signal was obtained by adjusting the proportion of solvent.Fe(III)-MOFs,NH2-MIL-88B(Fe),MIL-53(Fe),MIL-53(Fe)membrane,were in.situ grown on the surface of Fe3O4@RGO.Then magnetic graphene based Fe(Ⅲ)-MOFs derivative composites(NH2-MIL-88B(Fe)@Fe3O4@RGO,MIL-53(Fe)@Fe3O4@RGO,MIL-53M(Fe)@Fe3O4@RGO)membrane were obtained by calcining.With high electrochemical performance of composite materials,we simulated the activity of peroxidase and established non-enzymatic glucose electrochemical sensor to detect glucose with the detection limit of 0.072μ M.(3)A solvothermal method was used to prepare functional graphene composites(MFe2O4@RGO,M=Fe,Cu,Zn)with the best electrical signal,and then nHKUST-1 membrane was in situ grown on its surface.Functional fossil graphene nHKUST-1 derivative composites membrane,nHKUST-1 @MFe2O4@RGO,were obtained by calcining.Based on the high conductivity of composite membrane materials,we simulated the activity of peroxidase and the characteristics of glucose oxidase and established a non-enzymatic glucose electrochemical sensor to detect glucose.Because the nHKUST-1 membrane was spread on the surface of MFe2O4@RGO like a layer of membrane,the contact sites with glucose were increased,and thus the sensitivity of the electrochemical sensor was further improved with a detection limit of 8.1 nM which provided a new platform for the rapid and efficient detection of glucose.