Isolation And Identification Of Anaerobic Bacteria In Traditional White Wine Mud And Screening Of Functional Bacteria

Cellar mud is the basis in strong-flavor liquor production process.The categories and proportion of microbial populations in cellar mud have great impact on the flaour of white liquor.facultative bacteria is the main preponderant flora in cellar mud,so classificate and identificate them is necessary and establish the relation between their speciality and the flavour of white liquor.The main reason of high yield,low quality,high sale,low profit of Heilongjiang province white liquor lies in white liquor enterprises lack high qulity liquor cellar.Therefore,it is very important that research the structure,distribution and metabolites of microbial populations in the cellars of Heilongjiang white liquor industry,separate and select functional strains for improving the quality of Heilongjiang province white liquor and establish microbial database of liquor cellars.In this research,cellar mud samples that from different position of two different age cellars of fuyu old cellar spirit Co.,Ltd Heilongjiang Province were gather.The pure culture was obtained from the sample by classical microorganism separation and purification technology,statistics and analysis distribution information of anaerobe and facultative anaerobe in different cellar age and different position.Anaerobe were indentified to genus by traditionary and molecular biology identification method.Thereby,the diversity of microbial populations in the cellar mud were more comprehensive understanding.Selected cellar mud functional bacteria by physiological and biochemical indexes and gas chromatographic analysis(GC).It was known through analysised distribution information of anaerobe and facultative anaerobe in every layer of two cellar that:the amount of anaerobe are both over 30% of total bacterial count in two cellar;no matter total bacterial count,anaerobe amount and facultative anaerobe amount,every layer of II cellar all more than every layer of I;In two cellar,the more near to bottom of cellar,the more of kinds and amount.11 pure cultivation strains of anaerobe were obtained in cellar samples of two cellar,and to named them FB2,FB3,FB8,FB21,FB23,SB4,SB8,TB4,TB5,TB7,TB14,after indentified,FB2 was Clostridium sporogenes,FB3 and TB4 were Propionibacterium acnes,FB8 be likely to a new species of Clostridium,FB21 was Clostridium mesophilum,FB23 and SB4 were Clostridium bifermentans,SB8 was Paenibacillus anaericanus,TB5 was Clostridium sartagoforme,TB7 was Sporolactobacillus sp.,TB14 were Clostridium tyrobutyricum,Clostridium is dominant species amomg them.Use the content of ethanoic acid,butyric acid,hexanoic acid in fermenting liquor as index,selected 3 cellar mud functional bacteria by gas chromatography,and further study FB8 which was greatest functionality among 3 strains functional bacteria.GC/MS deteced tunning showed FB8 indicated more flavor component which were beneficial to Chinese liquor.