Construction Of Ultra-Sensitive Electrochemical Biosensor Based On The Nanocomposite Materials For The Detection Of ?2,3-Sialylated Glycans

The electrochemical biosensor has the advantages of high sensitivity,good specificity and simple operation,so it has important application in the fields of medicine,life sciences,environmental testing and food safety.With the rapid development of nanotechnology and emergence of novelty nanomaterials,the electrochemical biosensors have a better application prospect.In this paper,fabricate an biosensor with high selectivity,good stability and high sensitivity,which combined with the advantages of nanomaterials,electrochemical biosensor technology and use of specifically bind between the biomolecule.This biosensor was used to detect circulating blood ?2,3-salylated glycans,laying the experimental basis for clinical application.The detail works are as follows:Construction of ultra-sensitive electrochemical biosensor based on the nanocomposite materials for the detection of ?2,3-sialylated glycansThe expression of ?2,3-sialylated glycans is also different in the process of tumors development.The changes of ?2,3-sialylated glycans in blood can be used as important molecules markers for early diagnosis of cancer.In order to improve the sensitivity of electrochemical biosensor,the multi-walled carbon nanotube with good conductivity and the specific surface area was used as the modified material for glassy carbon electrodes.However,considering the poor dispersibility and easy agglomeration of multi-walled carbon nanotubes,the multi-walled carbon nanotubes were functionalized by polyamide-dendrimers and then chitosan was used to disperse the nanocomposites then modified the surface of the glassy carbon electrode.Maackia amurensis lectin immobilized on the surface of the electrode after used the homobifunctional cross linker 1,4-phenylene diisothiocyanate,Maackia amurensis lectin is an ideal tool that can specifically recognize ? 2,3-sialylated glycans.So specific detection of ?2,3-sialylated glycans in serum can be achieved.The method for the determination of ?2,3-sialylated glycans in this study can provide a reference for clinical and life sciences.The method is effective in the detection range of 10 fg m L-1 ~ 50 ng m L-1 of the linear response,the minimum detection limit of 3 fg m L-1(S / N = 3).Showing high sensitivity,good reproducibility and stability,in the clinical early diagnosis of the application shows a good application prospects.