Structure Design And Flow Field Study Of Single Cell Capture Microfluidic Chip

Single cell capture microfluidic chip has the advantages of low cost,high throughput,easy manufacturing,integration,non-toxic,good stability etc.Because of the characteristics,the cell capture microfluidic chip is increasingly becoming an important carrier on the study of life science and pharmaceutical analysis.It is an important promise of single cell analysis,pairing,fusion,disruption and analysis of intracellular components for capturing single cell.The capture which based on fluid dynamics method in the field of microfluidic chip is an important way to achieve and realise those operations that mentioned above.In the article,according to the micro scale theory of Stokes flow,turbulent flow and the two-dimensional steady laminar model,the stages of the single cell capture process analysed and a coupled mechanical model of cells,fluid and surface in single cell trap microfluidic chip built.Based on coupled mechanical model,analysis about the forces of single cell,the relationship between the forces and the velocity during the single cell capture process can be achieved,which laid the foundation for realising the controllability of single cell in the next step of the research.Based on the statistical theory,a method for studying the flow velocity of microfluidic chip proposed.Make full use of the existing equipment and conditions,the preliminary exploration to the single cell capture experiment was carried,understood and familiar with the PDMS microfluidic chip fabrication craft.On the basis of existing micro trap structure for single cell capture,a pillar,single slit,double slit micro trap stucture for single cell capture was proposed,the size of each micro trap structure is close to the size of the capture cell,and can only accommodate one cell in principle.Combined with the finite element analysis software,the paramenters of entrance velocity,micro trap unitstructure size and the distribution of micro trap arrays were studied by numerical simulation calculation,the velocity contours,the pressure contours of the fluid field in the micro trap and the total chip were discussed.Through the simulation experiment analysis of three types-six kinds of structure of single cell capture device,the most suitable single cell capture device:rectangular pillar single cell capture device was determined.Through the flow velocity analysis of different sections,the flow field uniformity of single cell capture array was verified.Through the analysis of the different micro trap mass flow,the flow variations in the different micro well structures at the entrance was obtained.Based on the analysis of the efficiency and the uniformity of the whole arrsys,the evaluation coeficient about single micro trap which mianly refers to the capture barrier,fluid field update factor,extremely low velocity region and so on,the structure of the whole chip was optimized,which provided a system and theoretical support for the design of the single cell capture microfluidic chip and laid the foundation of the study on the single cell capture in the future.