Design And Fabrication Of A Microfluidic Capture Chip For Lung Cancer Metastasis

Background:Lung cancer is one of the most common and malignant cancers worldwide.It is most often diagnosed at late stage,when it has already presented distal metastases.Metastasis remains the leading cause of lung cancer death.This fact highlights the need for further understanding of mechanisms that guide tumor cells migration in the microenvironment and critical metastatic steps,in order to develop more effective strategies in treating lung cancer.However,the study of cancer metastasis cannot go further due to the limit of traditional experiments.With the development of microfluidic chip technology,more and more scholars combine it with biological technology.Microfluidics has greatly promoted the development of cancer research,because of its many advantages such as high-throughput analysis,high sensitivity,multi-factors analysis,bionic simulation.Works:1.Through comprehensive and in-depth investigation of the research progress of lung cancer,we emphasized that EMT is hotspot and the focus today.Furthermore,according to the summary of currently experimental methods,we concluded that integrated microfluidic chip technology is an optimal experimental method.Therefore,we regard the EMT,a typical mechanism of lung cancer metastasis,as our main research content.2.Based on the principle of specific binding of antibody and antigen,we designed and fabricated a microfluidic capture chip by microfluidic technology in our lab.First,SU-8 mask and SPR-220 mask were manufactured by photolithography techniques.Then,PDMS was used as chip material and desired micro channel was produced with help of curing technology.Finally,the cover of PDMS was combined together with a glass substrate and microfluidic chip was obtained.The substrate of microfluidic chip was coupled with anti-N cadherin and anti-vimentin,which could capture the tumor cells by the antigen on the cell surface.Conclusions:1.We designed and fabricated a microfluidic chip to detect the occurrence of EMT and capture the lung cancer cells which undergo EMT.What's more,this chip is expected to provide support for clinical detection,and to explore mechanisms about interactions between cancer cells and microenvironment during the process of lung cancer metastasis.2.We optimized the fabrication process and identified the causes of the early failures.In a word,our works will create a positive condition for analysis in vitro of lung cancer cells after EMT.