Design And Implementation Of An Optical Projection Tomography System And Experimental Verification

Optical projection tomography(OPT) is a novel high-resolution three-dimensional imaging technology. The emergence of the technology fills the vacancy of quondam imaging devices in 1-10 mm imaging field of view. It provides a new technical means for life science research. As an emerging technology of modern imaging, with its high resolution, low cost, easy building, easy debugging and upgrading, especially with its extensive applicability, OPT has successfully won most scientists' favor.Under this background, on one hand, we presented an OPT imaging system with high resolution and high imaging quality, on the other hand, we explored ways to expand the application of our custom OPT system. The main works of this paper are as follows:1.OPT imaging system design and implementation. Based on the principle of easy to build and install, through theoretical analysis and a large number of experimental tests, we independently developed a set of an OPT imaging system combining motion and imaging. On hardware, a s CMOS camera with large target and low noise was employed as an OPT imaging device, which equipped with a high precision electronic control platform. The system was designed to construct a high accuracy, high resolution, and low noise optical imaging system. On controlling software, we developed a controlling software including multi-operation modes integrated with motion and imaging referring to software engineering standards, based on the QT and VTK. Filtered back projection(FBP) reconstruction method is used in the data processing in this system. In addition, according to the application requirements, we also completed the designing and processing of the mold and related equipment for experiments.2.Analysis of OPT system performance. Firstly, we tested, compared and analyzed the influence of core parts, such as different illuminants and microscopes, on the imaging system. This experiment and related discussion further demonstrated the feasibility of the key equipment package, including telecentric backlighting illuminant, Navita microscopes and science SCMOS. Then, we tested the spatial resolution of the system and compared the spatial resolution of the system on different magnifications factor using resolution charts. Finally, we tested the depth of field(DOF) and imaging effect of the OPT system. Under different magnifications, we took use of the DOF charts testing the value of DOF, and the fruit-fly experiment shows that the DOF has effects on the 3D reconstruction.3.Imaging application research based on the OPT system. In this section, we discussed in detail how sample being prepared and processed before OPT imaging of Arabidopsis siliques, which is also a model plant. At the same time, we sorted out an overall set of operation specification of experimental procedure, which has an excellent impact on final imaging.