The Design And Synthesis Of Dual-Functional Polymer Brushes And Their Derivatives For Biosensoring Study

The biosensor is a very important tool of technology research and engineering. It has wide application prospects in fermentation, environmental monitoring, food engineering, biomedical science, military medicine due to its high accuracy and sensitivity, specificity, detection of rapid response, simple operation, easy to carry, etc.An ideal biosensor should be used directly to detect a biological sample, so it can avoid the tedious process of separation and purification from samples. Some ingredients in biological samples (such as protein or cells) are often non-specific adsorption on the surface of a biosensor, forming the so-called "biofouling". These may decrease its specificity and other expected performance and even may completely destroy the biosensor. So it is of particular importance to develop anti-fouling biosensor.The purpose of this study is to solve the problem ofthe adsorption of non-specific protein on the surface of a biosensor at detecting process. The anti-protein polymer brushes were grafted from the substrate surface through atom transfer radical polymerization (ATRP), choosing mercaptan as initiator and poly(ethylene glycol) methacrylate as anti-protein material. Then, creating the active site of Biotin, investigating its specific recognition to Avidin. The characterization of samples were carried out by using X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR) and surface plasmon resonancespectroscopy (SPR).The results showed that after modified POEGMA polymer brushes grafting from Bare Au substrate by ATRP technique, the value of protein adsorption on surface was less than the detection limit, indicating to possess excellent performance in resisting protein pollution. What's more, Biotin active sites created can effectively identify Streptavidin molecules, The molecular layer is about 16 (?).