| Experimental purposes: sports fatigue has always been one of the core issues of sportstraining, exercise physiology, sports medicine research. Because a proper understanding ofexercise-induced fatigue can not only help athletes improve their athletic ability?but also canprevent various diseases caused due to fatigue and injuries. Leave sports fatigue meaningless?today's sports arena, competition is increasingly iferce amount?athletes who are trying to gobeyond human limits?creating a superior performance history. Therefore?athletes who canonly rely on "high-intensity?high-load” training methods to continuously improve athleticperformance. The higher the intensity?the more likely the body to produce fatigue? higherloads?fatigue duration will be longer. So exercise fatigue mechanism became one stagesports ifeld research hot spots. In this study, Bedford fatigue modeling program (minorchanges)?the use of multi-level Treadmill Exercise programs exercise fatigue experimentalrat model. Observing the movement of glucose in rats immediately fatigue?muscle glycogen?serum insulin?muscle lactate changes occur?the use of theoretical research exercisebiochemistry and cell molecular biology methods for activity in rat skeletal muscle glucosetransporter protein?gene expression and its surrounding indicators the changes werecompared and discussed the mechanism of glucose transporter in motion the process offatigue in order to further study the mechanism of sports fatigue on glucose transport dataprovide a theoretical basis.Experimental methods:8-week-old male Wistar rats30(Production License No.:SCXK (Gan)2011-0001)?weight173.2?7.9g?all rats were fed during the week of adaptation,out underweight, overweight and bad mental condition suited treadmill exercise?reservationsrequired20were randomly divided into: control group (10)?fatigue group (10). In this study,Bedford fatigue modeling program (minor changes), the use of multi-level Treadmill Exerciseprograms exercise fatigue experimental rat model. According to the following procedure,sport period of7days. Load level is divided into three: I class load:0°?15m/min?30min; IIclass load:0°?20m/min?30min; class III load:0°?25m/min?60mm, for6days. Exhaustiveexercise over the ifrst seven days in accordance with the procedure to Level III load.Immediately after exhaustive Sacriifced weight were measured, exhaustive time, serum insulin, glucose, muscle glycogen?muscle lactate (LD)?GLUT4protein content, differentvariations of GLUT4gene expression indexes of rats. Exhaustive criteria are: rat runningposture by the stomp equation becomes prostrate type, can not continue to remain in therunning end of the runway, and the acoustic and optical stimuli are not motivated to maintainan animal running. Normal rats cage quiet life?not sports. Treadmill Training rats were usingelectronic stopwatch measuring time [1].Experimental result:(l)In the whole experiment process, the spirit of the groups of ratsis in good condition, found no wool, stool form change, casualties and other adverseconditions. Groups of rats weight change but not statistically signiifcant (P>0.05).(2)Thefatigue model using Bedford (make a little change) the results showed that sports fatiguegroup rats BU (blood urea nitrogen) value increases obviously have statistical signiifcance(P<0.01).Through the observation of the behavior of rats comprehensive physiological indexhas reached the experiment we think rats the expected level of fatigue. Using multi-stage loadof rat treadmill exercise plan successful sports fatigue experiment model, which laid afoundation for further experiments.(3)To the end of the experiment, compared with controlgroup, the fatigue group rats blood glucose.(GLU),serum insulin levels had significantlyincreased(P<0.001). Compared with control group, muscle glycogen?fatigue group ratsskeletal muscle lactic acid (LD)declined obviously.(4)Compared with control group (P<0.01)?rat skeletal muscle fatigue group-quadriceps GLUT4protein content increased.(5)Comparedwith control group, group of rats skeletal muscle fatigue sarcomere length contraction shortersignificantly (P<0.01).(6)Compared with control group, the group of rats skeletal musclefatigue, quadriceps membrane GLUT4gene expression.Experimental conclusions:(1)Inthis study, Bedford fatigue modeling program (minor changes), the use ofmulti-level Treadmill Exercise programs exercise fatigue rat experimental model issuccessful.(2)Fatigue rats treadmill exercise to exhaustion weight loss.(3)Blood glucose lower motor fatigue, elevated insulin; decreased significantly in ratskeletal muscle glycogen, muscle lactate (4)Exercise fatigue can make the gene expression in rat skeletal muscle intracellularglucose transporter raised, raise the rate of glucose transport. |
PDF Full Text Request