Levels Of Sclerostin In Gingival Cervicular Fluid And Saliva And Its Effect On Bone Tissue Of Patients With Chronic Periodontitis

Background and objective: Chronic periodontitis is a chronic infectious disease of the periodontal tissue and is the most common form of periodontitis.It has been shown that the absorption of alveolar bone is one of the main pathological changes of periodontitis.Sclerostin is a secreted glycoprotein secreted by bone cells.However,the mechanism of sclerostin expression and bone resorption in chronic periodontitis remains unclear.The aim is to explore the role of sclerostin in the development and progression of periodontitis and possible mechanisms.Method: This study selected 30 patients with moderate-severe chronic periodontitis and 30non-periodontitis healthy subjects.We checked the full mouth condition,and recorded the probing depth(PD),clinical attachment loss(CAL)and bleeding on probing(BOP)in 6 sites of each tooth.The researchers collected gingival crevicular fluid samples and saliva samples.Enzyme-linked immunosorbent assay was used to detect the concentration of sclerostin,β-catenin,RANKL,and OPG.And analyzed the correlation with the periodontal clinical indexes.Result: 1 The periodontal clinical indexes of chronic periodontitis group were significantly higher than those of non-periodontal group(P<0.05).2 The concentration,total amount and calibration concentration of sclerostin,β-catenin,RANKL,OPG in gingival crevicular fluid and the concentration of sclerostin in saliva of chronic periodontitis group were significantly higher than the non-periodontal group(P<0.05).3 In gingival crevicular fluid and saliva,the concentration of sclerostin,β-catenin,RANKL,OPG were positively correlated with PD,CAL,BOP and gingival crevicular fluid volume(P<0.05).There was a negative correlation between sclerostin concentration and β-catenin and OPG concentration(P<0.05).There was a positive correlation between sclerostin concentration and RANKL concentration(P<0.05).4 The RANKL/OPG ratio and calibration ratio in gingival crevicular fluid and the ratio of RANKL/OPG in saliva of patients with chronic periodontitis were significantly higher than thosein non-periodontal health groups(P<0.05).Conclusion: The results indicate that sclerostin can be used as a potential chronic periodontitis biomarker and targeted drug design site.And sclerostin may affect bone tissue damage of chronic periodontitis through the Wnt/β-catenin pathway and the RANKL/RANK/OPG pathway.