The Effects Of Puerarin On Vascular Insulin Resistance And Cardiovascular Remodeling In Salt-sensitive Hypertention

ObjectiveIn this study,we discuss effect of puerarin on vascular insulin resistance and cardiovascular remodeling in salt-sensitive hypertention as well as the mechanism.Methods12 Dhal salt-sensitive rats were randomly divided into control,hypertention or puerarin-treatment group.DS rats were given a normal?0.5%Nacl,NS?or high salt diet?HS,4%Nacl?for 5 weeks,additional group ofDS ratswas pretreated with puerarin and NS for 10 days,then switch to HS plus puerarinfor 5weeks with gavage(300mg·kg^-1·day^-1).the systolic blood pressure?SBP?was measured by tail-cuff method.Endothelium-dependent relaxation?EDR?to acetylcholine and Insulin was determined using organ chamber bath.Expression of the protein in the aortic tissue was determined by Western blot analysis.ResultsCompared with normal salt rats,HS rats induced hypertention?SBP:194±5mmHg,p<0.05?,aortichypertrophy?31%,p<0.05?,hearthypertrophy?23%,p<0.05?,puerarin significantly reduced SBP?173±4 mmHg,p<0.05?,aortic and heart hypertrophy in DS rats.Puerarin also reduced aortic medial thickness and myocardial cell surface area in DS rats.Compared with control rats,HS rats impaired EDR with reduction in the protein expression of phosphor-eNOS at ser1177,phosphor-AKt at Ser 473 and increase in the expression of phosphor-IRS1?50%?,phosphor-JNK?37%?,phosphor-MAPK?heart62%,vascular32%?,NF-?B?75%?and TNF-??55%?.Puerarin improved EDR and reversed the changes in DS rats induced protein expression of above molecules.ConclusionsOur results demonstrate that in DS rats puerarin protects against endothelial dysfunction and end oagan damage with a mild reduction in SBP,puerarin improved vascular insulin resistance and cardiovascular remodeling in salt-sensitive hypertention.the mechanism may be associated with improvement of insulin-mediated AKt/eNOS phosphorylation and inhibition of NF-?B inflammatory pathway.