Studies On The Effects Of Puerarin On The Experimental Diabetes Insulin Resistance And Its Mechanisms

Objective: To investigate the effect of Puerarin (Pue) on the Experimental diabetes insulin resistance (IR) and its mechanism. Methods: IR mice model induced Streptozotocin (STZ) and Hydrocortisone Sodium Succinate (HCSS). Study the influence of Pue on fasting blood sugar, fasting serum insulin and sugar tolerance on normal mice and model mice. Inject vein small dosage STZ and high- fat feed induced IR rat model. Study the effect of Pue on insulin sensitivity on IR rat by sugar tolerance test and hyperinsulinemic euglycenic clamp technique (HECT). Probe into the mechanism of Pue by detecting the contents of plasma insulin, triglyceride (TG), total cholesterol (TC), tumor necrosis factor α (TNF- α )and Leptin (Lep). Rat hepatic cell (BRL) were incubated for 16 hour in high insulin condition to induce IR cell model. Study the effect of Pue on the glucose consumption and NO synthesis of hepatic cell. Results: Pue has little influence on fasting blood glucose of normal mice, but can lower blood sugar and serum insulin of diabetic mice by STZ induced and increase insulin sensitivity index significantly as well as improve IR by HCSS induced significantly. Pue can improve the glucose tolerance and glucose transformation rate of insulin, lower blood sugar and blood fat(TG,TC), decrease the contents plasma insulin, TNF- α and Leptin of IR rat induced by small dosage STZ and high-fat feet. Also Pue can increase glucose consumption of rat hepatic cell in high insulin condition through regulating NO synthesis as well as reducing IDE expression. Conclusion: Pue can improve IR of the Experimental diabetes by decrease blood sugar and serum insulin level to protect β cell and hepatic cell, as well as lower TG, TC, TNF- α , Leptin and adjust NO synthesis, reduce the expression of IDE gene to improve the insulin sensitivity of hepatic and periphery tissue.