| [Research purposes]Bronchial asthma is one of the most common respiratory diseases in childhood.According to incomplete statistics,there are currently about 200 million people in the world suffering from the disease.The prevalence in different countries ranges from 1% to 3%.The prevalence rate in developed countries is higher than that in developed countries.In China,the prevalence in cities is higher than in rural areas.According to the survey of prevalence of childhood asthma in urban areas of China,the prevalence of asthma in children is 1.97%,and the prevalence rate in two years is 1.54%.At present,70%-80% of asthma in children in China occurs before the age of 5,and about 20%.The patient has a family history [1].With the increase in the incidence of children with asthma,not only the family,the society has brought a huge burden,but also gradually evolved into a serious public health problems.If asthma is diagnosed and treated improperly during the onset of children,as the disease course will be extended As irreversible airway stenosis or airway remodeling and other pathological changes occur one after another,the prevention and treatment of asthma during remission are particularly important.Traditional Chinese medicine has obvious advantages in the clinical application of asthma remission.This research project mainly discusses the mechanism of action of Yiyi Ointment in relieving asthma in children from the perspective of cellular immunity.Object:(1)Observe the serum interleukin-4(IL-4)and interferon gamma(INF)before and after treatment in children with asthma-induced spleen-qi deficiency during the asthma remission stage in children with Yifei paste group and control group(montalukast sodium combined with budesonide)Regulate the concentration of INF-?;(2)compare the efficacy and safety of children with Yifei Paste and control groups for the children with asthma-relieved lung-spleping qi deficiency type before and after treatment to reduce TCM symptom scores and improve asthma control level Sex;(3)The levels of IL-4 and INF-?in the bronchoalveolar lavage fluid(BALF)were observed in each group,and the differences in IL-4 and INF-?levels in the treatment group and the control group were compared;(4)To explore the mechanism of Yifei ointment of children on asthma relief period.Methods: 1.Clinical study: 40 cases of TCM syndrome differentiation belonged to asthmatic children with lung-spleen-qi deficiency and were randomly divided into two groups(20 cases with montelukast combined with budesonide and 20 cases with children with Yifei paste).The indicators of these two groups of children,such as gender,disease duration,and age,were not significantly different from those tested.Children in the treatment group were given Yifei ointment.In the control group,montelukast sodium tablets were combined with budesonide.During the treatment,other drugs that affect the efficacy of observation were discontinued,and the dietary care of the children was taken care of.The serum levels of IL-4 and INF-? in the two groups of children were measured after the end of the 6-week treatment period.The level of asthma control and TCM syndrome scores were evaluated in the two groups of children.The treatment was completed at the end of treatment for 3 months,6 months,and one year later,compare their efficiency and conduct statistics.2.Experimental study: Balb/C strain female mice were randomly divided into 6 groups after a week of feeding,6 in each group.Except for the blank group,the remaining 5 groups were prepared by intraperitoneal injection and inhaled ovalbumin(OVA)sensitization method for remission asthmatic mouse model,divided into 6 groups,namely the model group,blank group and montelukast sodium joint deployment Neide group,children Yifei Gao low dose group,children Yifei cream medium dose group,children Yifei Gao high dose group.On the second day after the completion of the model,the blank group and the asthma model group were given normal saline,the montelukast sodium combined with the budesonide group,and the children's Yifei Gao low-,middle-,and high-dose groups were dosed,and each was given daily.Stomach once(inhalation of budesonide)for a total of 28 days after the last administration for 24 hours,observed the effect of different drugs on the level of IL-4 and INF-? in the bronchoalveolar lavage fluid of each group of mice.SPSS19.0 statistical software was used for statistical analysis of experimental data.Results:1.Clinical study: Serum IL-4 and INF-? concentrations were significantly modulated before and after treatment in both groups.The difference was statistically significant(P<0.05).The children's Yifei Gao group was significantly more effective than Montelukast sodium combined with budesonide in reducing TCM symptom scores and asthma control(P<0.05).2.Experimental study:(1)compared with blank group,asthma model group mice in the bronchoalveolar lavage fluid.The level of IL-4 was significantly increased,and the difference was statistically significant(P<0.05);montelukast sodium combined with budesonide group,children's Yifei Gao low-dose group,middle-dose group,high-dose group and model group The ratio of IL-4 was significantly decreased(P<0.05),and the difference was statistically significant.Kidney Yifei Ointment was significantly lower in IL-4 levels than in montelukast combined with budesonide group(P<0.01).(2)Compared with the blank group,the asthma bronchoalveolar lavage fluid in model group mice,The level of IFN-? was significantly lower in the Chinese population(P<0.01);montelukast sodium combined with budesonide,low-dose Yifei cream,children's Yifei cream middle-dose group,and children's Yifei Compared with the model group,the level of INF-? increased significantly in the high-dose group(P<0.01).The middle-dose group had a significant increase in IFN-? levels compared with the montelukast sodium and budesonide group.(P<0.01).Conclusion:(1)Kidney Yifei Ointment can reduce the level of IL-4 in children with asthma remission and up-regulate the level of INF-?.Compared with montelukast sodium tablet and budesonide group,it can significantly improve TCM symptom score and asthma control level.,Clinical observations show that children Yifei ointment is useful for the treatment of childhood asthma relief period,suitable for promotion.(2)Kidney Yifei Ointment can reduce the level of IL-4 in BALF of model mice and up-regulate the level of INF-?.The experimental results show that Yifei ointment has the function of regulating the cellular immune function of mice,and it is a clinical treatment for asthma in remission stage in children.Provides theoretical support. |
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