Influence Of Pang Shi An Tai Tang To The Blood Serum Level Of Tumor Necrosis Factor ? And Interleukin 10 On The Rat Of Threatened Abortion

Objective:The purpose of this is to fill the stomach to the pregnant rats Tradit ional Chinese Medicine(TCM)Pang Shi An Tai Tang and Western Medicine Dy drogesterone Tablets.Using Mifepristone simulation Threatened abortion rat,then through observation threatened abortion rats of general situation as mental state,and fur of color,and activities degrees,and has no vaginal bleeding and pregna ncy thing discharge.By comparing the respective group of pregnant rats' impla ntation number,and live birth number,and abortion number,and abortion rate.E xplain to the effect of Pang Shi An Tai Tang keeping pregnant to threatened a bortion rats.By comparing each group of pregnant rats' serum tumor necrosis fa ctor(TNF)-?,interleukin(IL)-10 content and changes of TNF-?/IL-10 ratio.Expl ore the mechanism of Th1(TNF-?)/Th2(IL-10)cytokines to threatened abortion and the influence of Pang Shi An Tai Tang to the balance relationship between the Th1/Th2 cytokines.Methods:Female and Male rats in accordance with 2:1 in the cage,by observing the female vaginal smear or pessaries select pregnant female rats 1 day.The pregnant 1 day pregnant rats were randomly divided into 6 groups for normal group,model group,Dydrogesterone group,Pang Shi An Tai Tang high,medium and low dose groups.By filling the stomach of pregnant rats 12 days of Western medicine,Traditional Chinese medicine.In addition to normal the other groups taken mifepristone,establishing the model of threatened abortion rats.Then observe the mental state of pregnant rats,fur color,activities,with or without vaginal bleeding,and so on.Executed each group of pregnant rats and observe the shape of uterus,then cut open uterus,observe the shape and size of fetal rats,carefully record the number of live births,bed number,number of stillbirths,calculating to the abortion rates.By enzyme-linked immunosorbenassay in detection of serum TNF-? and IL-10 content and the ratio of TNF-?/IL-10.Results:1.It is no difference between groups of pregnant rats implantation number(P>0.05).Compared with the normal group,model group significantly lower number of live births(P<0.05);Abortion and abortion rate of model group was obviously higher than that of normal group(P<0.05);The number of Pang Shi An Tai Tang medium dose group live births,number of abortion,abortion rate has no difference compared with normal group(P>0.05).The number of Pang Shi An Tai Tang high and low dose groups,Dydrogesterone group live births all lower than the normal group(P<0.05),number of abortion,abortion rate has no difference compared with normal group(P<0.05).Compared with the model group,The number of Pang Shi An Tai Tang high,medium and low dose groups,Dydrogesterone group live births all higher than the model group(P<0.05),number of abortion,abortion rate higher than model group(P<0.05).Compared with Dydrogesterone group,the number of Pang Shi An Tai Tang medium dose group live births higher than Dydrogesterone group(P<0.05),number of abortion,abortion rate lower than Dydrogesterone group(P<0.05);Pang Shi An Tai Tang high,low dose groups has no difference with Dydrogesterone group(P>0.05).The number of Pang Shi An Tai Tang medium dose group live births higher than the low dose group(P<0.05),It is no difference of Pang Shi An Tai Tang high compare with medium and low dose groups(P>0.05)?2.TNF-? content of model group serum and TNF-?/IL-10 ratio higher than other pregnant rats groups(P<0.05),IL-10 content lower than other pregnant rats goups(P<0.05).Compared with normal group,Pang Shi An Tai Tang medium dose group the concent of TNF-? and IL-10,TNF-?/IL-10 ratio has no difference(P>0.05);the concent of TNF-? and the ratio of TNF-?/IL-10 higher than normal(P<0.05).Compare with Dydrogesterone group,Pang Shi An Tai Tang medium dose group the concent of TNF-? and the ratio of TNF-?/IL-10 lower than Dydrogesterone group(P<0.05);Pang Shi An Tai Tang high,low dose groups has no difference with Dydrogesterone group(P>0.05).Pang Shi An Tai Tang medium dose group the content of TNF-? lower than the low dose group(P<0.05),It is no difference of Pang Shi An Tai Tang high compare with medium and low dose groups(P>0.05)?Conclusion:Pang Shi An Tai can improve the number of live births,and reduce the number of and the rate of abortion.Bying reduce the content of TNF-? of threatened abortion rats serum,improve the content of IL-10 and the ratio of TNF-?/IL-10,make the balance of Th1(TNF-?)/Th2(IL-10)in the direction Th2 shift,so as to achieve the purpose of keeping pregnant.