| Purpose: By observing Chu Shi Antai Fang general rat model of threatenedabortion, live births, number of abortions, abortion rate to confirm the impact ofChu Shi Antai Fang tocolysis efficacy; By detecting Chu Shi Antai Fang forthreatened abortion serum tumor necrosis factor in rats (TNF)-α affect the levelof interleukin (IL)-10from T helper cell (Th) l/Th2(TNF-α/IL-10)-typecytokines angle investigate Chu Shi Antai Fang, Depth study of the mechanismChu Shi Antai Fang to provide an objective basis for therapeutic advantage ChuShi Antai Fang.Methods: Take estrus females mated with males2:1overnight. The next morningassay using vaginal smear examination by one female, were found in the sperm orvaginal suppository as first day of pregnancy, pregnancy one day the rats wererandomly divided into five groups of12.2threatened abortion with mifepristonebuild model rats, the establishment of a high Yangzhai Antai Recipe, low-dosetreatment group, progesterone group and the normal group was the control group.3rats in each group were sacrificed on day9, the removal of the uterus anduterine cut observation point and form a complete implantation, bright andtransparent embryo count calculates the number of abortion, miscarriage rates;abdominal aortic blood Determination of serum TNF-α in rats by enzyme-linkedimmunosorbent assay, IL-10levels.Results: Normal group, progesterone group, Yangzhai Antai Recipe high-dosegroup, the number of live births Yangzhai Antai Recipe low-dose group wassignificantly higher than the number of live births in the model group, there weresignificant differences (P <0.01); no significant difference (P>0.05) compared tothe high dose group Yangzhai miscarriage, Yangzhai Antai Recipe low dose group, the number of live births progesterone group pairwise; progesteronegroup, high dose group Yangzhai miscarriage, the number of abortions YangzhaiAntai Recipe low-dose group, the abortion rate was significantly lower than thenumber of abortions in the model group, the abortion rate, there is a significantdifference (P <0.01); Yangzhai miscarriage high dose group, Yangzhaimiscarriage no significant difference (P>0.05) compared to the low dosagegroup, the number of abortions progesterone group, the abortion rate in pairs..2TNF-α levels in serum of model group was significantly higher than the rest ofTNF-α levels in serum of rats, there is a significant difference,(P <0.05);Yangzhai miscarriage high dose group, Yangzhai miscarriage Fang low dosegroup, TNF-α levels of progesterone group difference was no significantdifference between two (P>0.05).3serum IL-10levels in the model group wassignificantly lower than serum IL-10levels in each group of rats, there is asignificant difference,(P <0.01); Yangzhai miscarriage high dose group, lowYangzhai Antai Recipe dose group, progesterone group IL-10levels twenty-twowas no significant difference (P>0.05).Conclusion: For threatened abortion with mifepristone-induced rat model,Yangzhai miscarriage order to improve the general situation in rats, increase thenumber of live births, reduce the number of abortions and abortion rate,confirming the Yangzhai Antai Recipe from the macroscopic effect. Yangzhaimiscarriage side can reduce the serum levels of TNF-a, IL10-level raise andlower the ratio of Th1/Th2make Th1(TNF-α)/Balance Th2(IL-10) to Th2direction to achieve security the purpose of the tire, resulting in cytokine levelsconfirmed Yangzhai Antai Recipe inhibit adverse maternal immune response, theefficacy of prevention and treatment of threatened abortion. |
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