Inducing Effects Of HGF On Inwvasion And Expression Of VEGF-C In MGC-803 Gastric Cancer Cells Through AKT Signaling Pathwags

Objective : To investigate the effect of HGF on invasion and expression of VEGF-C in MGC-803 cells and to explore the role of PI3K/ AKT signaling pathway in HGF-induced invasion and expression of VEGF-C in MGC-803 cells?Methods:1)The gastric cancer MGC-803 cells were treated with different concentrations(0,10,20,40,80 ng / ml)HGF for different time(12,24,48 h),MTT method was used to observe the effects of HGF on the proliferation of MGC-803 cells,and the appropriate concentration and time of action were screened out.2)After treated with different concentrations of HGF and PI3 k / AKT inhibitor LY294002,The expression of VEGF-C,AKT,P-AKT protein in MGC-803 cells was detected by Western Blot.3)After treated with different concentrations of HGF and PI3 k / AKT inhibitor LY294002,The invasion of MGC-803 cells was detected by Transwell invasion assay.Results:1)HGF significantly increased the proliferation of gastric cancer MGC-803 cells in vitro,In a certain period of time,with the increase in HGF concentration,The proliferation ability of gastric cancer MGC-803 cells significantly enhanced,With the prolongation of the time,the proliferation ability of MGC-803 cells increased first and then decreased,and the proliferation ability of MGC-803 cells was thestrongest at 24 h.2)HGF promote the expression of VEGF-C and P-AKT in MGC-803 cells,but had no significant effect on the expression of AKT,And the expression of VEGF-C and P-AKT protein was the highest at 40 ng / ml HGF,After treated with LY294002,The expression of VEGF-C and P-AKT was decreased significantly,but the expression of AKT was not changed,Suggesting that HGF can promote the expression of VEGF-C in gastric cancer MGC-803 cells by activating AKT signaling pathway.3)HGF can significantly promote the invasion of gastric cancer MGC-803 cell in vitro,At the concentration of HGF 40 ng/ml,the invasive ability of MGC-803 cell was best,After treated with LY294002,The invasion ability of MGC-803 cells was significantly decreased,Suggesting that HGF could promote the invasive ability of MGC-803 cells by activating AKT signaling pathway.Conclusion :HGF promotes the expression of VEGF-C and invasion of gastric cancer MGC-803 Cells through PI3 K / AKT signaling pathway.