PGE₂ Increases Invasion Of Gastric Cancer Cells Via The EGFR/ERK2 Signaling Pathway

Objective:1. To identify whether PGE₂ can promote adhesion, migration and invasion of human gastric cancer cell line MKN28 in vitro.2. To investigate the transcription of VEGF mRNA and expression of VEGF protein in human MKN28 gastric cancer cell line after treated with PGE₂.3. To investigate the role of EGFR and ERK2 signal transduction pathways in any effect exerted by PGE₂ on the adhesion, migration and invasion abilities and on VEGF expression of human MKN28 gastric cancer cell line.Methods:1. The cell adhesion ability was tested by MTT assay, cell migration ability was identified by Transwell plate and invasion ability was tested by a Matrix gel coated Transwell plate.2. Extrinsic source PGE₂, ERK specific inhibitor PD98059 and EGFR specific inhibitor AG1478 were added to culture media and the changes of cell adhesion, migration and invasion were then tested to determine whether EGFR signaling pathway or ERK pathway was involved in the the mechanism exerted by PGE₂.