The Preliminary Study On The Transcription Factor FOXP2 Involved In EMT Process Of Breast Cancer

Breast cancer is the most common malignancy in women.The incidence of breast cancer continues to increase with age and the trend is getting younger in the world,which caused serious damage for female body and mental health.Early treatment of breast cancer mainly take minimally invasive surgery,if the patient9 s physical condition is not allowed,they can only take conservative treatment.In the face of poor metastases,early recurrence,and poor prognosis in human breast cancer,if we implement targeted intervention early,the poor prognosis of breast cancer may be improved effectively.Epithelial-Mesenchymal Transition(EMT)refers to the biological process of translucent polar cells transformed into loose interstitial cells,which plays an important role in tumor metastasis.EMT is a complex process involving multiple signaling pathways that requires special transcription factors to participate.In the transfer process,epithelial cells to obtain a higher plasticity to promote tumor metastasis.FOXP2 transcription factor belongs to the fork head box transcription factor family,which is the earliest human discovery and language related genes and plays an important role in human language learning ability and facial movement.FOXP2 has a certain role in lung tissue,cardiovascular,intestinal and nerve tissue development.In addition,FOXP2 expression in osteosarcoma,rectal cancer and gastric cancer can inhibit the EMT process of tumor cells.This article focuses on the role of FOXP2 in the process of breast cancer EMT.The relationship between FOXP2 and EMT markers was found by clinical samples.The model of breast cancer EMT was identified as:MCF-7 and MDA-MB-231.Then,we transfected pcDNA3.1-his-FOXP2 to breast cancer interstitial MDA-MB-231 cell,and expressed FOXP2,finding that the expression of interstitial marker Vimentin decreased,the expression of epithelial marker E-cadherin increased and the migration ability of cells decreased.In order to further verify that FOXP2 inhibits the migration ability of breast cancer epithelial cells,we used the lentivirus way to make FOXP2 gene silence,and then through the flow sorting technology to screen stable silencing FOXP2 breast cancer MCF-7 cell lines,we found that the expression of epithelial marker E-cadherin decreased,the expression of interstitial marker Vimentin increased and the migration ability of cells increased.This shows that FOXP2 is an important inhibitory factor in breast cancer cell EMT.It was found that FOXP2 gene silencing enhanced the proliferation ability and cell plate cloning ability of MCF-7 cell line,which indicated that FOXP2 was also a key factor to inhibit the proliferation of breast cancer cells.