Mechanistic Study Of The Role Of CEBPB In Regulating Triple Negative Breast Cancer Cell Proliferation And Migration

Objective: In this study,BT549 cell line,which belongs to triple negative breast cancer(TNBC),was used to investigate the effects of CCAAT/enhancer binding protein beta(CEBPB)on the proliferation,migration and invasion of BT549 cells,and to preliminarily explore the relevant molecular mechanism.Methods: BT549 cell line with stable knockdown of CEBPB gene was constructed by lentivirus,and the knockdown effect of lentivirus was detected by Real-time PCR and Western Blot at the level of gene and protein,respectively.CCK-8 assay was used to detect the effect of knocking down CEBPB gene on the proliferation of BT549 cells.Colony formation assay was used to detect the effect of knocking down CEBPB gene on the colony formation ability of BT549 cells.The effect of knockdown of CEBPB gene on S phase in BT549 cells was detected using an EdU kit.The effect of knocking down CEBPB gene on wound healing ability of BT549 cells was detected by cell scratch test.The effect of CEBPB gene knockdown on the invasive ability of BT549 cells was detected by cell invasion assay.By analyzing transcriptome sequencing results,we obtained the expression data of CEBPB knockdown cells and control cells,and studied the molecular mechanism of CEBPB regulating TNBC.Results: BT549 cell line with stable knockdown of CEBPB gene was successfully constructed.The results of cck-8 showed that the ability of cell proliferation was significantly reduced after the knockdown of CEBPB gene.The results of colony clone formation showed that it was difficult for cells to form colonies after the knockdown of CEBPB gene.The EDU assay showed that the number of cells in S phase decreased after the knockdown of CEBPB gene(P < 0.01).The results of cell scratch test showed that the ability of cell scratch healing was significantly reduced after the knockdown ofCEBPB gene(P < 0.01).The results of cell invasion showed that the invasion ability of CEBPB gene was significantly reduced(P < 0.01).The results of transcriptome sequencing showed that MAPK signaling pathway was inhibited by knockdown of CEBPB gene.Conclusion: There is a close relationship between CEBPB and TNBC,and CEBPB is highly expressed in TNBC.When CEBPB gene was knocked down,the proliferation,migration and invasion ability of BT549 cells decreased.The knockdown of CEBPB gene may mediate the decline of proliferation,migration and invasion of BT549 cells by inhibiting MAPK signaling pathway.