Regulation Of Lung Progenitors Proliferation In Idiopathic Pulmonary Fibrosis

Objective: Application in molecular biology and cell biology to investigate the effect of fibroblasts on regulating lung progenitors proliferation in idiopathic pulmonary fibrosis.Method:1.Lung cell suspension was prepared from β-actin-GFP mice.2.Lung progenitors were obtained by Fluorescence activated Cell Sorting and co-cultured with lung fibroblast cells from either idiopathic pulmonary fibrosis patients or normal individuals.3.The fibroblasts were treated with TGF-β receptor inhibiter SB431542,determination of FGFl and FGF2 changes.4.The lung progenitor cells in mice wiht mice lung fibroblasts secrete FGF1,FGF2,FGF1/FGF2 mixture traind,the expression and the effect of FGF1,FGF2,FGF1/FGF2 mixture on lung progenitors proliferation were observed.Result:1.The cloning efficieney of lung progenitors.when co-cultured with normal lung fibroblast cells for 8 days,was(3.5±1.1)%,while the cloning efficiency was reduced to(0.04±0.04)% when co-cultured with lung fibroblasts from idiopathic pulmonary fibrosis patients.The difference between the 2 groups was statistically significant(P<0.05).2.Application of TGF-β receptor inhibitor SB431542 in mice lung fibroblasts of 24 hours,SB431542 increased lung fibroblast growth factors FGFl and FGF2 expression.FGFl m RNA expression was increased to the experimental group 0.0055 from 0.0002 in the control group.FGF2 m RNA expression of the amount raised to the experimental group 0.00015 from 0.0008 in the control group.The data of the two groups were statistically significant,but there was no significant change in the morphology of the lung fibroblasts.3.After FGFl、FGF2 and FGFl/2 was co-cultured with lung progenitors for 8 days,the cloning efficiency of lung progenitors had statistical signficance,FGF1、FGF2 及FGF1/2 promoted the growth of lung progenitors.Conclusion: During the development of idiopathic pulmonary fibrosis,fibroblast secreted FGF l and FGF2 regulate lung progenitors proliferation.