| BackgroundIdiopathic pulmonary fibrosis(IPF)is the most important subtype of idiopathic interstitial pneumonias(IIP).The Pathophysiology are mainly Usual interstitial pneumonitis(UIP).And it is continuously progressive.The onset of IPF is insidious,the etiology and pathogenesis are still unclear.and the diagnosis needs to combine clinical-pathological-imaging,which is difficult to diagnose.At present,there is no effective treatment for IPF.With poorly prognosis and highly mortality,The median survival time after diagnosis is only 3-5 years,which brings great burden to families and society.The purpose of this study is to detect the expression of TGF-beta 1 and STAT1 in serum of IPF patients,and to analyze the correlation between them and clinical parameters such as inflammatory markers,lung function,blood gas analysis(BG)and high resolution CT(HRCT),so as to explore their role in the pathogenesis and severity assessment of IPF,and to provide new ideas for the diagnosis and treatment of IPF.MethodForty patients with IPF who were admitted to the Department of Respiratory Medicine,Second Hospital of Shandong University between June 2016 and December 2018 were selected as the case group.At the same time,twenty-two healthy subjects under physical examination at the Physical Examination Center of the Second Hospital of Shandong University were recruited as the control groups.Collect and organize basic clinical data of two groups of subjects,including gender,age,smoking history,etc.Fasting venous blood was collected from IPF patients and healthy controls.Serum levels of transforming growth factor-beta(TFG-betal)and signal transducers and activators of transcription 1(STAT1)were measured by enzyme linked immunosorbent assay(ELISA).The specific procedures are strictly in accordance with the requirements of the kit.Patients in the IPF group completed basic clinical examinations within 24 hours after admission,such as white blood count(WBC),neutrophil percent(N%),and C-reactive protein(C-reactive protein,CRP),arterial blood gases such as partial pressure of oxygen(Pa02),partial pressure of carbon dioxide(PaC02),oxygen saturation(Sa02),and lung function such as forced expiratory volume in one second/forced vital capacity(FEV1/FVC),total lung capacity(TLC),diffusion function DLco and chest high resolution CT(HRCT),etc.At the same time,the healthy control group also completed tests for WBC,N%,CRP and lung function.Data analysis was performed using SPSS 22.0 statistical software.Result1.The serum levels of TGF-?1 and STAT1 in patients with IPF were higher than those in healthy controls(P<0.05).2.Correlation analysis showed that the serum levels of TGF-?1 in the IPF group were positively correlated with STAT1 levels(P<0.05).3.Correlation analysis showed that the serum levels of TGF-?1 in the IPF group were negatively correlated with TLC(%),DLco(%),and Pa02(P<0.05);positively correlated with N%,PaC02,and HRCT scores(P<0.05).There was no significant correlation with WBC,CRP,FEV1/FVC(%),and smoking index.The level of serum STAT1 were negatively correlated with TLC(%)and DLco(%)(P<0.05);positively correlated with WBC,PaC02,HRCT scores(P<0.05);and there were no significant correlation with CRP,N%,FEV1/FVC(%),Pa02 and smoking index.Conclusion1.The serum levels of TGF-?1 and SATA1 in IPF patients were higher than those in healthy controls,suggesting that these two cytokines may be involved in the pathogenesis of IPF.2.Correlation analysis showed that there was a certain correlation between serum TGF-?1,SATA1 levels and clinical indicators such as inflammatory index,lung function,blood gas analysis and HRCT in patients with IPF,suggesting that TGF-?1 and SATA1 may be used as a reference index for assessing the severity of disease in patients with IPF. |
PDF Full Text Request