Study Of Vaginal Microenvironment And Human Papilloma Virus Infection For Patients With Cervical Intraepithelial Neoplasia

Objective Analyse the vaginal micro-ecological condition and humn papilloma virus(HPV)infection for patients with cervical intraepithelial neoplasia(CIN)before and after loop electrosurgical excision procedure(LEEP),to provide a date for restoring vaginal micro-ecological condition in the treatment of CIN.Methods Retrospective analysis has proceed in patients who was diagnosed CIN with HPV infection and treated by LEEP from Jan.2014 to Dec.2016 at the Second Affiliated Hospital of Chongqing Medical University,evaluating their vaginal microflora and HPV infection before and after LEEP.Result 845 patiens with complete follow-up date.Patients with vaginal microflora imbalance accounted for 81.5%(711/845),the pathogens can be clearly diagnose accounted for 37.2%(314/845),the incidence of bacterial vaginosis(BV),vulvovvaginal candidiasis(VVC),trichomonas vaginitis(TV)and aerobe vaginosis(AV)were 26.4%(223/845)、6.9%(59/845)、2.5%(21/845)、1.7%(14/845),respectively,the incidence of BV was significantly higher than the incidence of other pathogens(P<0.05).After LEEP treatment,233 of the 845 patiens was positive for HPV persistently,and vaginal microflora imbalance accounted for 80.7%(188/233),for those HPV positive,the incidence of BV and VVC was 37.3%(87/233)and 6.9%(16/233),respectively.The rest of the 612 patiens was negative for HPV,vaginal microflora imbalance accounted for 14.4%(88/612),the incidence of BV and VVC was 6%(37/612)and 1.3%(8/612),respectively.The incidence of imbalanced vaginal micro-ecological,BV and VVC was significantly higher in patients with HPV positive than patients with HPV negative post-LEEP(P<0.05).Conclusion The imbalanced vaginal micro-ecological is prevalent in CIN patients with HPV infection,and the BV is the most common pathogens.HPV persistently infection for CIN post-LEEP is related to vaginal microflora imbalance and pathogens infection such as BV and VVC,which indicate that is very inportent to restore the vaginal micro-ecological banlance and clear the pathogens during CIN treatment.