Studies On The Activation Effect Of Crude Polysaccharide From Arenaria Kansuensis On Murine Immunocyte In Vivo And Vitro

In this study,we first extracted crude polysaccharide from arenaria kansuensis.We examined the activation of AKCP on the proliferation and function of immune cells in mice,by in vitro experiments.And then through the detection of different doses of AKCP immunosuppressive mice immune indicators to be verified.In vitro and in vivo studies have confirmed that AKCP has an immune promoting effect.In vitro studies,mice peritoneal macrophages and spleen lymphocytes were cultured in vitro and be treated by AKCP in different concentrations for 48 hours.The lymphocyte proliferation and NK killing activity were detected by MTT method,the CD3~+/CD4~+/CD8~+ subsets in spleen lymphocytes were analyzed by FCM,and the levels of IL-2 and IFN-? in spleen cell culture supernatant were tested by ELISA.The results showed that after high,middle and low concentration AKCP treatment,the phagocytic and NO secreting activities of peritoneal macrophages,the proliferation index as well as IFN-? secretion level of lymphocytes,and the NK killing activity were significantly higher than those in control group(P<0.05).In middle AKCP group,The CD3~+,CD4~+ subset in spleen lymphocytes,the level of IL-2 secreted by lymphocytes was obviously increased(P<0.05).In vivo studies,mice were randomly divided into five groups: control group,cyclophosphamide(CTX)group and three AKCP in high,middle and low-dosages groups.In addition to control group other groups' mice received CTX i.p.to establish immunosuppressive model.After AKCP were administrated by i.p for 10 days,the mice were killed,and the weight gain rate,thymus and spleen index,Spleen lymphocyte proliferation as well as CD3~+/CD4~+/CD8~+subsets,NK killing activity,phagocytosis activity of peritoneal macrophages,levels of IL-2 and IFN-? in serum were detected.Results showed that Compared with control group,in addition to NK killing activity,other items in CTX group were all significantly decreased(P<0.05).AKCP in different-dosage treatment could relieve those effects.In high and middle-dose AKCP group,the weight gain rate,thymus index,CD3~+ and CD3~+CD8~+T cells in spleen,phagocytosis activity of macrophage were significantly higher than which in CTX group(P<0.05).In low-dose AKCP group,the spleen index,lymphocyte proliferation index,and the levels of IL-2 and IFN-? in serum were obviously higher than which in CTX group(P<0.05).The above results show that,AKCP has abilities to activate the proliferation and function of murine immunocyte in vitro;In vivo,AKCP could protect immune functions of immunosuppression model mice induced by CTX,including lymphoid organ weight,lymphocyte proliferation,Th1 cytokine secretion and even monocyte-macrophage function.