| Objective: To analyze the animal model of periodontitis in the construction of the detecti on rate of P.gingivalis in Apo E-/-mice in the aorta,and the activation of P.gingivalis VE Cs innate immune signal transduction related receptor,NF-kappa B transcription factors a nd the regulation mechanism and the effect on the proliferation and migration of VSMCs,in order to further explore the possible role of As in the occurrence and development of P.g ingivalis and related mechanisms and the correlation between As and the significance of th e research,and provide new ideas for the prevention and treatment of early As.Methods: Under anaerobic conditions,P.gingivalis was cultured and identified with Gra m staining.The Apo E-/-mice periodontitis model was established by ligaturing the maxilla ry second molar tooth and the coating bacteria method.control group was no ligation and o nly coated with PBS in tooth neck.Control group and experimental group were fed in high fat diet.The mice were killed after 12 weeks and the aorta and bilateral maxillary alveolar bone were retrieved.The content of P.gingivalis in aorta were detected by 16 S r RNA poly merase chain reaction and were analyzed with semi quantitative technique.The atheroscler otic plaque formation was detected by Frozen sections of aortic tissue,HE staining and oil red O staining,Immunohistochemical staining the TLR-2 and TLR-4 expression and NF-k appa B nuclear translocation and VSMCs proliferation and migration.were detected on the VECs cell surface.Results:(1)In this experiment,the cultured P.gingivalis on the BHI blood agar plate sho wed the characteristic of black round smooth colony,and under the oil microscope,it was G-,which was a pure culture.It can be used for following experiments.(2)Periodontitis was found in the experimental group mice maxillary second molar mes ial and distal proximal soft tissue retraction,alveolar crest reduced,severe alveolar bone re sorption;HE staining showed that the epithelial surface were erosive,the proliferation of th e epithelial and the proliferation of collagen fiber hyperplasia in periodontal tissue.The al veolar bone resorption was observed under the microscope,and the periodontal tissues of t he maxillary second molars were severely damaged in the experimental group,and the alve olar bone was absorbed down to the apical 1/3,but there was no abnormal change in contro l group.(3)The presence of P.gingivalis was detected in all aortic specimens,but the content of P.g ingivalis in the experimental group was significantly higher than control group(P < 0.01).(4)The aortic HE staining and oil red O staining results showed that the experimental grou pformed more a typical As aortic plaque,lipid content,and plaque areawas significantly gr eater than control group(P < 0.05).(5)The immunohistochemical staining showed that the VSMC aorta from media to intima proliferation and migration intensity in experimental group were significantly higher than c ontrol group,the range of the endothelial cells of TLR-2,TLR-4 and NF-?B strong positiv e expression was significantly higher than control group.Conclusion: The P.gingivalis of periodontal infection may promote the occurrence and d evelopment of As in aorta of Apo E-/-mice by affecting the expression of TLRs and NF-ka ppa B and the proliferation and migration of smooth muscle cells. |
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