Study On Pharmacodynamic Of Sanfengjiebiao Granules-preparation Evaluation

Objective: Through animal experiment,to observe the Sanfengjiebiao Granules in fever,cough,phlegm,relieving asthma,anti-inflammatory effect.To investigate the efficacy of Sanfengjiebiao Granules in asthma,expectorant,antitussive and anti-inflammatory effects,and provide experimental basis for clinical application.Methods: The establishment of fever in rats induced by dried yeast,observe the antipyretic effect of Sanfengjiebiao Granules.Mice cough induced by ammonia spray method and the cough of guinea pigs with citric acid spray method,record the cough frequency and cough latency of experimental animal.To observe the antitussive effect of Sanfengjiebiao Granules.The trachea phenolsulfonphthalein and capillary tube method,by recording the excretion of phenol red expectoration and rats,observe Sanfengjiebiao Granules expectorant effect.To establish an experimental model of guinea pig asthma induced by histamine spray and antispasmodic experiment Sanfengjiebiao Granules on isolated guinea pig tracheal smooth muscle intact,to observe the antiasthmatic effect.Observe the effect of sanfengjiebiao on the degranulation of mast cells and the inhibitory effect of croton oil on rat ear swelling induced by croton oil,to study the anti-inflammatory and anti allergic effects.Results: The heating effect of Sanfengjiebiao Granules can significantly inhibit the yeast induced in rats after administration of 1 h,the antipyretic effect can last for a long time.There was a certain correlation between the strength of the antipyretic effect with the dose of Sanfengjiebiao Granules.Sanfengjiebiao Granules high dose group could significantly prolong the ammonia induced cough in mice was significantly reduced(P<0.001),citrate induced number of guinea pig cough(P<0.001),dose effect relationship strength.The phenol red and glass capillary tube experiments,the results showed that Sanfengjiebiao high dose group can significantly increase phenolsulfonphthalein excretion quantity of trachea(P<0.001),significantly increased sputum effect(P<0.001),and the expectorant effect and the positive control drug is ammonium chloride.Histamine spray induced asthma model of guinea pigs,Sanfengjiebiao Granules showed high and low two dosage groups could significantly prolong the latent period of asthma induced by histamine(P<0.001),caused byhistamine phosphate in isolated guinea pig tracheal contraction was significantly inhibited(P<0.001).The mastocyte degranulation test and found that the Sanfengjiebiao Granules high dose group and low dose group can significantly inhibit mastocyte degranulation,anti allergic effect,and high dose of Sanfengjiebiao Granules group compared with the control group had significant difference(P<0.001),the low dose group compared with the control group there is also a significant difference(P<0.01),dose effect relationship strength.Each dose group Sanfengjiebiao Granules significantly inhibited the ear edema induced by croton oil,after inflammation 4 h markedly,the high dose group have significant difference(P<0.01),low dose group has significant difference(P<0.05),showed that the anti-inflammatory effect of strength and a certain dose dependent relationship.Conclusion: Through the experimental study,Sanfengjiebiao Granules has obvious antipyretic effect on fever,ease and effect in the treatment of cough,obvious expectorant effect of expectoration;has strong antiasthmatic effect,the effect of anti inflammation and anti allergic effects.The above effects of Sanfengjiebiao Granules were correlated with the dose of drugs.