Clinical Value Of Quantitative Detection Of Serum ANCA And ASCA In Patients With UC

Objective:By detecting the level of ANCA(Anti-neutrophil cytoplasmic antibodies,ANCA)and ASCA(Anti-saccharomyces cerevisiae Antibodies,ASCA)in the ulcerative colitis(UC)group,the disease control group and the healthy control group,To explore the significance of diagnosis,differential diagnosis,the expression of disease severity and curative effect assessment of UC by single and combined detection of each index.Methods:105 UC patients from the ward and outpatient department of the First Affiliated Hospital of Shanxi Medical University were enrolled as UC group,and all the diagnosis of UC met IBD diagnostic standard of the Chinese Medical Association meeting in Guangzhou.52 non-UC patients which were diagnosed as other intestinal disease(i.e.colitis,terminal ileitis,intestinal tuberculosis,intestinal polyps,white’s disease and crohn’s disease),according to the colonoscopic examination,were enrolled as disease control.Meanwhile,100 healthy volunteers were enrolled in this study as healthy control.The levels of serum ANCA-IgG,ASCA-IgA and ASCA-IgG were measured with enzyme-linked immunosorbent assay(ELISA).And the levels of ANCA-IgG,ASCA-IgA and ASCA-IgG from some patients of UC group were measured.Results:1.ANCA-IgG serum level in UC group、the disease control group and the healthy control group were 17.53±30.59U/mL、4.03±5.96U/ml and 3.15±2.24U/mL;positive rate were 61.9%、13.46% and 6%.Both the serum ANCA-IgG level and its positive rate in UC group were significantly higher than those in non-UC disease control group and healthy control group(p < 0.01).2.The serum ASCA-IgA,ASCA-IgG level and the positive rates in UC group had no statistial differences when compared with those in non-UC disease control group or thehealthy control group(p > 0.05).3.The sensitivity of serum ANCA+,ANCA+/ASCA-and ASCA+/ANCA-in UC group was 61.9% and 55.2%,respectively,whereas the specificity was 91.45% and 94.08%.The sensitivity of serum ASCA+and ASCA+/ANCA-in the disease control group was 5.33%and 3.85%,respectively,whereas the specificity was 83.9% and 88.78%.4.The ANCA-IgG level was associated with the severity of UC when it was divided as mild,moderate or severe sub-groups,and the differences of ANCA-IgG level among the three groups were statistically different(p < 0.05).They are respectively 4.65±2.81U/mL,14.72±18.41U/mL and 63.99±52.10U/mL.But there was no correlation between the level of ASCA-IgG or ASCA-IgA and the severity of UC.5.The level of ANCA-IgG,ASCA-IgG or ASCA-IgA were not correlated with the locations of the UC occurrence.Conclusion:1.Quantitative determination of serum ANCA was helpful forthe UC diagnose and helpful forestimating UC serious level and the treatment effect.2.Combined quantitative determination of serum ANCA-IgG,ASCA-IgG and ASCA-IgA was helpful for the UC differential diagnosis.