| Background: Sevoflurane inhalation anesthetics is commonly used in clinical anesthesia,induction of fast,high controllability is the precondition of its wide application,has been widely used in various surgical anesthesia.So the effects of sevoflurane on each viscera function also concern.Perioperative stress caused by trauma to the study of myocardial injury is the anesthetic doctor concerned topic,by low temperature,step-down method has achieved good control effect,the anesthetic itself whether there exist the anesthesia benefits worth exploring research.Apoptosis is a biological metabolism of the body's organs start on the cell update of metabolic changes,and the manner of death of outside intervention reasons have a significant difference between the two,is a biological process of physiological change,is to maintain exuberant vitality,cells,tissues and organs but not every cell apoptosis is a physiological process.Under normal physiological condition between cell proliferation and apoptosis to maintain a dynamic change,to maintain the body's internal a relatively dynamic stability has an important role.Some scholars put forward before the myocardial cells and the central neurons apoptosis will not occur,but in the early 1990 s,some scholars have found these so-called body formed in the process of differentiation of the late cells can also have the apoptosis process of myocardial apoptosis from a severe arrhythmia in patients with severe cardiomyopathy myocardial found evidence,prove the myocardial in certain cases,including drugs,environment,etc.,can appear the myocardial apoptosis,and find the answer.From more and more clinical and basic research experts in the research,the result of the myocardial cells must be participating in the development of the disease process,also in the process of the occurrence of various diseases and outcome plays a different role,a variety of diseases may be necessary for the change process of one of the basic cell;Most perioperative patients with internal diseases,cardiac function of attention and evaluation has been a top priority,from the psychological stress to traumatic stress,patients often reflected in the cardiovascular system,previous research on myocardial apoptosis damage mechanisms and related factors,genes are more.With the development of national economy,therefore,cardiovascular disease prevalence is also gradually rise,development to reduce apoptosis gene and antiapoptotic drugs,biological products will be for the early prevention of heart disease and myocardial ischemia reperfusion and a variety of reasons cause cardiovascular wall damage has an important role,has important clinical significance.The apoptosis of myocardial cells of the micro basic research,prevent or delay the myocardial cell apoptosis is valuable,anesthesia doctor find professional breakthrough point is very important.Objective: The establishment of chronic inhalation of low concentrations of seven halothane in rat model.The concentration of sevoflurane concentration were higher than in operating room.detection of myocardial cell apoptosis in rats in each period,each time the sea was detected in myocardium of rats with IL-18,NO,and its clinical significance in myocardial apoptosis.Methods: 2-4 weeks old healthy male SD rats,weight 80-120 g.Were randomly divided into four groups,24 rats in each group,control group and sevoflurane group,sevoflurane inhalation concentration at which is divided into 30 ppm,100ppm,300 ppm group.Sevoflurane group daily inhaled sevoflurane concentration corresponding to 6 hours of continuous inhalation for 2 weeks,4 weeks,8 weeks,12 weeks,the control group inhaled equivalent air,Richard I.Mazze reference method before the start of the application of the formula,molecular weight concentration = molecular weight ×22.4 × absolute temperature / inhalation volume in the experimental box(in which the concentration of the quality and volume of the unit,respectively ppm,mg,m3)load into sevoflurane calculated to experimental exposure box,in order to achieve the experimental concentration in rapid sevoflurane concentration,and the application of gas chromatography gas concentration detection box steady.Under the same conditions,the control group only received medical compressed air.4 groups of animals were continuously inhaled for 6 hours a day for 2 weeks,4 weeks,8 weeks and 12 weeks.Detection of ECG in different periods and to keep the heart specimens.HE staining was used to determine the changes of myocardial cell number;immunohistochemical detection of IL-18 and NO protein expression in heart tissue;the expression by reverse transcription enzyme reaction and Western blot analysis set IL-18,in the myocardium in a rat model of NO gene and protein.Results:1.Cell morphological observation,under the high power lens,myocardial cells can be clearly seen,have obvious different groups of cells express,air suction set no apoptosis occurred four time points,30 ppm sevoflurane group,no obvious changes of myocardial cell morphology,four time points(2 weeks,4 weeks,8 weeks,12 weeks)no obvious apoptosis occurred;100ppm sevoflurane group,2 weeks,4 weeks,8 weeks does not appear,apoptosis,12 weeks myocardial cell apoptosis,statistical analysis,there is statistical significance,P = 0.038;300ppm sevoflurane group,2 weeks,4 weeks of rats did not appear obvious apoptosis phenomenon,8 weeks and 12 weeks when the cell apoptosis,between groups,and the comparison group were statistically significant,P < 0.01.2.Comparison,electrocardiogram(ECG)in rats,the concentration of 100 ppm 12 weeks rats abnormal ECG found that different degree,and under the concentration of 300 ppm,abnormal ECG and after eight weeks of rats,and part of the ecg of myocardial ischemia,and in about 30 ppm concentration exposure environment,has not abnormal ECG in rats.3.The immunohistochemical detection of myocardial cell IL-18,NO expression in the two groups have significant differences,the early 30 ppm concentrations,molecular biological detection of myocardial tissue in rat,IL-18 is slightly lower than normal control group,and NO concentration slightly higher than the normal group,was statistically significant,P < 0.01,there is NO special change after 4 weeks,100 parts per million in the group,12 weeks experiment,IL-18 significantly higher than the other three time points and comparison between groups was statistically significant,NO obvious lower than the other three periods,comparison between groups have statistical significance;In 300 ppm group,IL-18 positive cells expression rate significantly better than 8 weeks,12 weeks,4 weeks,2 weeks two time points,and comparison in the group have statistical significance between groups,P < 0.05,and NO expression in 8 weeks and 12 weeks,express declined obviously,within the group and the comparison between groups were statistically significant,P < 0.05;Controls the air inhaled group IL-18 NO difference,NO expression.4.In myocardial tissue for RT-PCR and Western Blot test results and the varying degree change,the basic trend is consistent with immunohistochemistry and HE staining results.Conclusion: The long-term inhalation of low concentrations of sevoflurane in rats of myocardial cell apoptosis phenomenon,with the increase of the concentration of inhalation,the extension of time,performance is more obvious;the long-term inhalation of low concentrations of sevoflurane in rat myocardial tissue IL-18,NO expression was significantly different,apoptosis in myocardium of rats by one of the important. |
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