The Effects Of Sevoflurane Pretreatment On MiRNAs And Inflammatory Factor In A549 Cells And Lung Cancer Patients

Objectives:Lung cancer is one of the most common malignancies,and its mortality rate is the leading cause of death in all cancers.miRNA is involved in various cellular activities,such as growth,development,stress response,apoptosis and carcinogenesis.miRNA can be used as oncogene or tumor suppressor gene.Sevoflurane has protective effects on multiple organs,as well as the role of anti invasion and migration of cancer patients,but the effect of on lung cancer cell miRNA is not fully understood.Therefore,this study investigated the protective effect on lung cancer patients with sevoflurane,clear the effects of different concentrations and different time of sevoflurane preconditioning in A549 cells and patients with non-small cell lung cancer the changes in the amount and type of miRNA and the relationship between inflammatory factor and miRNA,to provide the basis for the use of sevoflurane in patients with lung cancer.Methods(1).After being cultured for 24 hours,A549 cells were divided into two groups: control group(group C)and sevoflurane group(group SEVO).Sevoflurane group were pretreated with 1% and 3% sevoflerane 1h,2h.To detect the content of miRNA34 a,146a,223,155,21,221 and Bcl-2 protein in the A549 cells of the two groups,and the apoptosis rate of cells was detected by RT-qPCR;(2).20 cases of non-small cell lung cancer patients,ASA I-II,were randomly divided into control group(group C,10cases)and sevoflurane group(group SEVO,10cases),two groups received the same anesthesia induction program,SEVO inhaled 3% sevoflurane for 30 min,one lung ventilation after sevoflurane preconditioning,infusion of propofol and remifentanil intravenous anesthesia to the end of operation.SEVO group,before induction of anesthesia(T1),sevoflurane preconditioning 30min(T2),and C group,before induction of anesthesia(T1),30 min after intravenous anesthesia(T2),detect the contents of serum mi RNA155,146 a,21;(3).The groups are the same as the second part: 20 cases of non-small cell lung cancer patients,were randomly divided into two groups,and the two groups after induction,C group received propofol and remifentanil total intravenous anesthesia maintenance,3% SEVO group with the same method after induction,Inhalation of 3%sevoflurane 30 minutes,one lung ventilation after sevoflurane preconditioning,infusion of propofol and remifentanil intravenous anesthesia maintained until the end of operation.The levels of mirna155 and IL-8 were detected before anesthesia induction(T1),sevoflurane pretreatment 30min(T2),and after one lung ventilation(T3).Results(1)The expression levels of miRNA in 1h and 2h,there was no significant difference between the 1%SEVO group and the C group(P>0.05).3%SEVO group compared with C group,the miRNA155 were significantly decreased in 1h and 2h.(P<0.05);miRNA146,there was no significant difference between the two groups in 1h(P>0.05),but increased significantly after 2h in 3%SEVO group(P<0.05);miRNA34a,there were no significant difference between the two groups in 1h(P>0.05);miRNA21,Similarly,there were no significant difference between the two groups in 1h(P>0.05);but decreased significantly after 2h in 3%SEVO group(P<0.05);miRNA221,223,there were no significant differences between the two groups(P>0.05);The apoptosis rate of SEVO group was significantly higher than C group and the anti-apoptotic protein Bcl-2 were significantly lower than C group(P<0.05);(2)Compared with the T2 time points,the serum miRNA155 level of 3%SEVO group was significantly lower than T1 time points(P<0.05);miRNA146a and miRNA21,there was no significant differences between T1 and T2 time points.(3)The levels of serum miRNA155 and IL-8,there was no significant differences between the two groups in T1 times point(P>0.05).In C group,there was no significant differences in T2,T3 and T1 time points(P>0.05).SEVO group,in T2,T3 time point,the contents of serum miRNA155,IL-8 was significantly lower than T1 time point(P<0.05).Conclusion3% sevoflurane can regulate the expression of miRNA in A549 cells and lung cancer patients,and increase the apoptosis rate of lung cancer cells,however,one percent of the sevoflurane did not have this effect.Sevoflrane pretreatment could inhibit the expression of serum miRNA155 gene in non small cell lung cancer patients and decrease the expressionof inflammatory cytokines,which could be used safely in the operation of lung cancer patients,inhibit the proliferation and metastasis of tumor and improve the survival rate of patients.