The Significance Of Serum Levels Of S100B Protein And NSE In Patients With Migraine

Objective:Migraine is a common disease.The pathogenesis of migraine is not completely understood yet.Recent studies have suggested that migraine may be a progressive disease and not a benign disease considered as before.Migraine repeated attacks may cause neural cells damage.S100B which is a protein marker of glial damage or activation,and neuron specific enolase(NSE)which is a marker of neuronal damage are getting more and more attention in clinical research.To further understand the pathogenesis of migraine and explore whether brain damage is presented in migraine,we investigated serum levels of S100B protein and NSE in patients with migraine and analyzed the correlation of serum levels of S100B,as well as NSE,with headache characteristics,including disease duration,duration,frequency,the score of VAS and HIT-6.Methods:1 In total,53 migraineurs who fulfilled the criteria of International Classification of Headache Disorders beta version,including 20 cases of migraine with aura and 33 cases without aura were consecutively enrolled in this cross-sectional prospective study,conducted in the Department of Neurology of The 2nd Hospital Of Hebei Medical University from March 2015 to December 2016.50 healthy subjects were chosen as control group in the Medical Examination Center of The 2nd Hospital Of Hebei Medical University at the same time.Detailed clinical data of all patients were collected,including general data,clinical data and epidemiological data.2 Blood samples(5 ml)were collected during a headache-free period from a peripheral arm vein into a standard biochemistry tube.All blood samples were centrifuged and the supernatant was immediately removed,then stored at-80 ? until assayed.Enzyme-linked immunosorbent assay was used to detect the serum levels of S100B protein and NSE.3 we used SPSS 20.0 software to compare the differences between migraine group and the controls,female patients and males patients,MA group and MwoA group respectively and analyzed the correlation of serum levels of S100B,as well as NSE,with headache characteristics,including disease duration,duration,frequency,the score of VAS and HIT-6.Results:1 Serum levels of S100B were significantly higher in migraineurs than controls(P<0.01).There was no significant difference in serum levels of NSE between migraine group and control group(P>0.05).2 There was no significant difference in serum levels of S100B between migraineurs with aura and without aura(P>0.05).Although the serum levels of NSE were higher in migraineurs with aura than that without aura,such a quite difference did not reach statistical significance(P>0.05).3 There were no significant differences in serum S100B and NSE between men and women in healthy controls(P>0.05).There were no significant differences in serum levels of S100B and NSE between men and women in migraine patiens(P>0.05).4 There was no significant correlation between serum levels of S100B protein,as well as NSE,and headache characteristics,including disease duration,duration,frequency,the score of VAS and HIT-6.Conclusions:Our results suggest that the activation of glial cells and dysfunction of blood-brain barrier are present in the migraine interictal period.There is no definite neuronal damage during the migraine intericval period.