| Objective: Through the study of the Th17 percent in the peripheral blood of the Graves patients and the negative control persons,and research on the different content of the prevotella bacteria and the Segmented Filamentous Bacteria between two groups to explore the relationship between the pathogenesis of the Graves disease and two kinds bacteria.Methods: 30 Graves disease patients and the same number of the negative control persons were divided them into GD group and NC group.Colleting the peripheral blood and their fetal as the samples.Using the DNA extraction kit to extract the total DNA of the fetal,and designing the special primers to amplificate the DNA of the SFB and prevotella through the technique of q PCR,to compare the content of the two groups.The percent of the Th17 was detected with the technique of flow cytometry and compare the difference bwtween two groups.Results: The percent of the Th17 cells in the peripheral blood of the onset Graves disease is much higher than it in the negative control groups,(12.46+3.14 vs.7.05+2.12;P<0.05),and the percentage of the Th17 cells is positively correlated with serum FT3,FT4(r=0.682,r=0.567,P<0.05),and have no relationship with others items.The content of the SFB of the Graves disease group is about 2.384×103times as much as its in NC Group,and the content of the Prevotella in the Graves disease group is about 1.825×103times as much as its in NC Group.Conclusions: 1.Th17 cells percentage in peripheral blood of the onset Graves disease were significantly higher than which in the negative group,and with a positive relationship with the FT3 and FT4 in serum.2.The content of the SFB and Prevotella in the intestinal flora with the onset Graves disease were much more than whicht in the negative group. |
