Investigate The Function And Possible Mechanisms Of MiR-145 On The Invasion Of Glioma U251 Stem Cells

Objective:To investigate the function and possible mechanisms of miR-145 on the invasion of glioma U251 stem cells.Methods:Primary glioblastoma cells were separated and cultured,which were transfected with lentiviral vectors for miR-21 mimics or miR-21 inhibitors for upregulation or downregulation of miR-145 expression levels.After that,cells were directly injected subcutaneously into the flanks of nude mice.The invasive effects of primary glioblastoma cells and stem cells were measured by Transwell assay,invasion related genes were screened,by mRNA array,and the expressing levels of matrix metalloprotease MMP-2,MMP-9,RECK and TIMPS were identified by Western blot.Results:miR-145 mimics transfection in vitro could effectively improve the expression of miR-145 in primary glioblastoma cells and stem cells,miR-145 inhibitor transfection could effectively inhibit the expression of mi R-145.Results showed that miR-145 mimics or inhibitor had no effects on the invasion of primary glioblastoma cells in vivo,and did not affect the expression of MMP-2,MMP-9,RECK and TIMPS.Conclusion:miR-145 expression is not related to the invasion properties of primary glioblastoma cells in vivo.